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Researcher in zero gravity with a tube-like device floating in front of her.
Aerial view of a desert area. Fire emerges from bottom of rocket rising from launchpad. Smoke and dust billow skyward.
Illustration of Low-Earth Orbit Flight Test of an Inflatable Decelerator (LOFTID).

Flight Testing Gene Expression,
Thermal Protection Technology

FLIGHT SUMMARY
NASA’s Flight Opportunities program supported the testing of two technology payloads on the August 29, 2024, suborbital flight test operated by Blue Origin. Part of the agency’s Space Technology Mission Directorate, Flight Opportunities rapidly demonstrates space technologies through suborbital flight testing with industry providers.

One payload was a University of Florida technology that helps researchers understand the biological effects of changes in gravity by analyzing patterns of gene expression in plant samples. Principal investigator Robert Ferl conducted this experiment himself, representing the first time a NASA-funded researcher is flying with their payload on a suborbital rocket. This flight test was funded by a grant through the Flight Opportunities program’s TechFlights solicitation with additional support from NASA’s Division of Biological and Physical Sciences.
 
The other payload is evaluating the ability of new materials for TPS (thermal protection systems) — developed by HeetShield of Flagstaff, Arizona, under the SBIR (Small Business Innovation Research) program — to withstand the rigors of launch and descent. Mounted to the outside of New Shepard’s propulsion module, the materials are exposed to spaceflight and reentry conditions, gathering valuable insights ahead of future mission applications.
 
Below is more about these payloads and the flight test objectives.

Learn more about how to access flight tests about Flight Testing Gene Expression,
Thermal Protection Technology

Flight Provider

Blue Origin

Flight Test Platform

Reusable suborbital rocket system

Launch Date

August 29, 2024

Location

Launch Site One, West Texas

University of Florida’s Gene Expression Analyses

Researchers seek to close the knowledge gap about how organisms initiate responses to changes in gravity.

About the Payload
Building on a long history of suborbital and orbital flight testing, this researcher-tended experiment aims to measure the biological response of plants to changes in gravity. Before flight, University of Florida researchers pre-loaded small, self-contained  Kennedy Space Center fixation tubes (KFTs) with plants and preservative. During the flight, principal investigator Rob Ferl activated the tubes to rapidly release a preservative at four key stages of gravity: 1 g before liftoff, at the start of microgravity (i.e., just after boost), at the conclusion of microgravity, and at the end of the flight. After the flight, Ferl and co-principal investigator Anna-Lisa Paul use transcriptome analyses — which capture the patterns of gene expression during the stages of flight — of the preserved plants to examine the effect of gravity transitions on the plants.

Applications
Using KFTs during suborbital flight could enable the collection of biological data in transitional gravity environments, which would shed light on our understanding of how various stages of spaceflight affect gene expression. Technology end users include biological researchers interested in capturing molecular structural changes in an organism in variable gravity environments. KFTs used suborbitally could also have applications in material sciences and fluid physics.

Flight Test Objectives
The flight test aims to evaluate the effectiveness of using KFTs to capture biological responses during the transitions from Earth’s gravity, through increasing g loads, to the microgravity environment enabled by Blue Origin’s New Shepard suborbital rocket system. The gene expression data collected is anticipated to characterize the early phases of the rapid adaptation that accompanies transitions among g loads.

The University of Florida’s technologies and experiment operations to evaluate plant molecular responses have been matured through flights aboard aircraft flying parabolic profiles as well as autonomous and crew-tended flight tests aboard suborbital rocket-powered vehicles. These innovations include telemetric imaging systems that can capture real-time signal transduction within plant organs, in addition to technologies that enable rapid plant preservation in KFTs to chemically “freeze” the moment for gene expression profiling, like the payload slated for this upcoming flight. Ferl and Paul have also gathered valuable data through testing aboard the International Space Station that has contributed to their technology’s development. The researcher-tended operations on the August 29, 2024, flight leveraged Ferl’s familiarity with the payload and experiment objectives as well as his extensive flight test experience.

Learn more about this payload and flight test
Researcher strapped into seat depresses plunger on tube. Window view is the darkness of space.
During an August 29, 2024, commercial suborbital flight supported by NASA’s Flight Opportunities program, University of Florida’s Rob Ferl conducted a hands-on biological experiment using a Kennedy Space Center Fixation Tube.
Blue Origin

For media inquiries, please contact:
Jasmine Hopkins | jasmine.s.hopkins@nasa.gov