Crystal growth mechanisms associated with the macromolecules adsorbed at a growing interface - Microgravity effect for self-oscillatory growth - 2 (Ice Crystal 2) - 08.29.18

Overview | Description | Applications | Operations | Results | Publications | Imagery

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Science Objectives for Everyone
The Ice Crystal 2 investigation examines the growth rates, and stability, of ice crystals in supercooled water that contains antifreeze glycoprotein (AFGP).The growth of ice crystals in the supercooled water is controlled by the preferential accumulation of the AFGP molecules at the surface where ice and water interface in solution. The growth of ice crystals, and their adsorption of AFGP, is observed in the microgravity environment, free from the effects of gravity-based convection.
Science Results for Everyone
Information Pending

The following content was provided by Yoshinori Furukawa, Ph.D., and is maintained in a database by the ISS Program Science Office.
Information provided courtesy of the Japan Aerospace and Exploration Agency (JAXA).
Experiment Details

OpNom: Ice Crystal 2

Principal Investigator(s)
Yoshinori Furukawa, Ph.D., Hokkaido University, Sapporo, Japan

Etsuro Yokoyama, Gakushuin University, Tokyo, Japan
Naohisa Ogawa, Hokkaido Institute of Technology, Japan
Nada Hiroki, AIST, Japan
Gen Sazaki, Ph.D., Hokkaido University, Sapporo, Japan
Ken Nagashima, Hokkaido University, Japan
Akitoshi Asakawa, Hokkaido University, Japan
Shunichi Nakatsubo, Hokkaido University, Japan

Information Pending

Sponsoring Space Agency
Japan Aerospace Exploration Agency (JAXA)

Sponsoring Organization
Japan Aerospace Exploration Agency

Research Benefits
Information Pending

ISS Expedition Duration
March 2013 - September 2014

Expeditions Assigned

Previous Missions
Ice crystal experiment was conducted in Increment18.

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Experiment Description

Research Overview

  • Ice crystal morphologies growing in a supercooled antifreeze protein (AFP) solution are drastically modified from those growing in a supercooled pure water, and the self-oscillation of growth is also observed. Based on the results of ground experiments, a model is proposed to explain these phenomena. It is called the 2-step reversible adsorption inhibition model, and must be confirmed by careful experiments. The crystal morphology may be strongly be affected by  convection, which cannot be prevented under  gravity. This effect can be prevented, and the effects of AFP obtained for ice growth under ideal microgravity experimental conditions in the Kibo Module of ISS.

  • Various images of ice crystals grown under supercooling temperatures are scheduled to be taken and data analyzed in hopes of clarifying the morphological changes and the time-sequence growth rates.

  • This research project illustrates the principle of the growth model for ice in the AFP solution, and opens the way for new research fields related to the fundamentals of crystal growth mechanisms controlled by biological macromolecules.


Information Pending

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Space Applications

Antifreeze glycoproteins have been discovered in fish living in polar regions. Ice crystals grow in a dramatically different fashion in the presence of this protein, compared to ice crystals that form in pure super-cold water. Scientists have not been able to successfully demonstrate how these proteins work, because ice crystal formation is likely also affected by convection (induced by gravity). Studying the interaction between ice crystals and antifreeze glycoprotein in microgravity could help researchers describe how the protein adheres to water molecules to stop the formation of ice.

Earth Applications

Biological antifreeze is found in several species of polar fish, where it prevents the formation of ice crystals even in freezing water. Understanding how antifreeze glycoprotein works could lead to new research in the fundamental physics of crystal growth, and how biological molecules can affect crystal formation.

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Operational Requirements and Protocols
Information Pending

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Decadal Survey Recommendations

Information Pending

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Results/More Information

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Results Publications

    Furukawa Y, Yokoyama E, Yoshizaki I, Tamaru H, Shimaoka T, Sone T.  Crystal growth experiments of ice in kibo of ISS. International Journal of Microgravity Science and Application. 2014; 31(3): 93-99.

    Furukawa Y, Nagashima K, Nakatsubo S, Yoshizaki I, Tamaru H, Shimaoka T, Sone T, Yokoyama E, Zepeda S, Terasawa T, Asakawa H, Murata K, Sazaki G.  Oscillations and accelerations of ice crystal growth rates in microgravity in presence of antifreeze glycoprotein impurity in supercooled water. Scientific Reports. 2017 March 6; 7: 43157. DOI: 10.1038/srep43157. PMID: 28262787.

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Ground Based Results Publications

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ISS Patents

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Related Publications

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Related Websites

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image NASA Image: ISS038E002242 - View of Ice Cell 2 installation.
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image NASA Image: ISS038E002237 - View of the Ice Crystal 2 experiment in the Ryutai (fluid) Rack.
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