Studying the Processes of Culturing Various Types of Cells (Kaskad) (Kaskad (Cascade)) - 03.14.18

Overview | Description | Applications | Operations | Results | Publications | Imagery

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Science Objectives for Everyone
The Studying the Processes of Culturing Various Types of Cells (Kaskad) investigation studies the processes of culturing cells of microorganisms, animals, and humans in microgravity to obtain concentrated biomass with a high concentration of cells producing a high output of target biologically active substances. The impact of flight factors on the properties of fungal culture cells and products of their biosynthesis was studied in order to achieve the maximum possible output of biomass and target biosynthesis products, i.e. biologically active substances. Work was performed on the fungal culture Mycelium radicis, a producer of the biostimulator substance of plant growth.
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The following content was provided by Tatiana K. Krasheninnikova, and is maintained in a database by the ISS Program Science Office.
Experiment Details

OpNom:

Principal Investigator(s)
Tatiana K. Krasheninnikova, Institute for Applied Biochemistry and Machine Building (Biohimmash), Russia

Co-Investigator(s)/Collaborator(s)
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Developer(s)
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Sponsoring Space Agency
Russian Federal Space Agency (Roscosmos)

Sponsoring Organization
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Research Benefits
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ISS Expedition Duration
April 2009 - September 2010; March 2011 - September 2015

Expeditions Assigned
19/20,21/22,23/24,27/28,29/30,31/32,33/34,35/36,37/38,39/40,41/42,43/44

Previous Missions
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Experiment Description

Research Overview
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Description
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Applications

Space Applications
During Kaskad, the properties and characteristics are studied of a fungal strain grown through culturing in orbital flight in a liquid nutrient medium, and optimizing the process parameters of placing a planting material and culturing cells in an enclosed bioreactor. As a result of the work, proposals and comments are formulated on science equipment operation, time and process parameters of conducting the experiment, which in turn lead to the creation of standard on-station biotechnology equipment to conduct stages of the biotechnological process of obtaining biologically active substances in orbital space flight.

Earth Applications
No earth application has been identified for this experiment.

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Operations

Operational Requirements and Protocols
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Decadal Survey Recommendations

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Results/More Information

A culture liquid of the fungal culture Mycelium radicis was obtained from the Kaskad experiment during ISS-36 and ISS-38. Based on the laboratory investigations of the culture liquid, it was concluded that the specimen obtained during these missions did not differ visually from the control sample. The fungal culture obtained in flight during ISS-37 was in a suppressed state while returning to Earth and lost its viability. The cause of this could have been bacterial contamination discovered in the culture liquid.

During laboratory investigations of the biochemical indicators of the culture liquid obtained during expeditions 36 and 38, a decrease was noted in all values in the flight sample compared to the ground counterpart obtained by culture according to the space experiment timeline and a control sample grown in a flask. This effect may be related to the impact of various spaceflight factors on the rate of metabolism of the fungal culture, resulting in a more intensive consumption of substrate components of the nutrient medium. During orbital flight while growing the fungal culture in the bioreactor, culture liquids were obtained with a balanced complex of biologically active substances that, at a specific incubation of working solution, manifest maximum growth stimulating activity for both roots and stems.

The most balanced complex of biologically active substances in the culture liquid obtained during expedition 36 was identified at a working solution dilution of up to 10 ppm. In the space experiment, a culture was obtained for the first time whose growth stimulating activity for stems and roots exceeded by 10 fold compared the indicator in the ground culture, and whose growth stimulating activity was higher than the control culture. In the culture liquid obtained in expedition 38, the optimal ratio of growth stimulating activity for roots and stems was observed at a working solution concentration of up to 1 ppm. For a statistically justified finding on the levels of biotechnological indicators and growth stimulating activity based on substances obtained from flight culture liquids, additional sessions of the Kaskad experiment will be carried out in the future.

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Related Websites
Energia - Science Research on the ISS Russian Segment

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Imagery