Microbial Dynamics in International Space Station - I (Microbe-I) - 12.28.16
The Microbe-I experiment monitors microbes on board the ISS which may affect the health of crewmembers. Science Results for Everyone
Scientists are monitoring the abundance and diversity of fungi and bacteria in the Japanese Experiment Module of the International Space Station (ISS). Samples are collected and analyzed periodically after the module is installed to the ISS. Microbe detection sheet (MDS) and swab culture tests of orbital samples and examination by electron microscopy are methods to detect microbes. This research and monitoring contribute not only to safety on long-duration spaceflight, but also to development of a standard microbial sampling method for the pharmaceutical and food industries, better hygiene in other closed habitats, and, potentially, better water quality control. Experiment Details
Koichi Makimura, Ph.D., M.D., Teikyo University, Tokyo, Japan
Masao Nasu, Osaka University, Osaka, Japan
Ichiro Sato, Ph.D., Teikyo University, Tokyo, Japan
Yayoi Nishiyama, Teikyo University Institute of Medical Mycology, Toyko, Japan
Takashi Q. Yamazaki, Ph.D., Japan Aerospace and Exploration Agency, Tsukuba, Japan
Yuji Tsukii, Hosei University, Tokyo, Japan
Takashi Sugita, Ph.D., Meiji Pharmaceutical University, Tokyo, Japan
Kosuke Takatori, Center for Fungal Consultation, Kanagawa, Japan
Yoshimi Benno, Riken, Saitama, Japan
Ryutaro Izumi, JAEA, Japan
Shinko Yamaguchi, Osaka University, Japan
Takashi Baba, Ph.D., Osaka University, Osaka, Japan
Toru Shimazu, Japan Space Forum, Tokyo, Japan
Kana Kuriyama, Japan Space Forum, Tsukuba, Japan
Katsuji Tani, Ph.D., Osaka Otani University, Osaka, Japan
Kazuaki Ichijo, Osaka University, Japan
Sponsoring Space Agency
Japan Aerospace Exploration Agency (JAXA)
Japan Aerospace Exploration Agency
ISS Expedition Duration
April 2009 - October 2009; March 2010 - September 2010
- The purpose of this experiment is to monitor microbes in Kibo which may affect the health of crewmembers. And the monitoring of the stress from microbes to crewmember is evaluated as a space medical impact.
Operational Requirements and Protocols
Decadal Survey Recommendations
Information Pending^ back to top
Ichijo T, Yamaguchi N, Tanigaki F, Shirakawa M, Nasu M. Four-year bacterial monitoring in the International Space Station—Japanese Experiment Module “Kibo” with culture-independent approach. npj Microgravity. 2016 April 21; 2: 16007. DOI: 10.1038/npjmgrav.2016.7.
Satoh K, Nishiyama Y, Yamazaki TQ, Sugita T, Tsukii Y, Takatori K, Benno Y, Makimura K. Microbe-I: fungal biota analyses of Japanese experimental module KIBO, international space station which passed for about 460 days. Microbiology and Immunology. 2011 December; 55(2): 823-829. DOI: 10.1111/j.1348-0421.2011.00386.x. PMID: 21950271.
Ichijo T, Yamaguchi N, Nasu M. Bacterial monitoring in the International Space Station – “Kibo”. Journal of Disaster Research. 2015 December; 10(6): 1035-1039. DOI: 10.20965/jdr.2015.p1035.
Ott CM, Pierson DL, Shirakawa M, Tanigaki F, Hida M, Yamazaki TQ, Shimazu T, Ishioka N. Space Habitation and Microbiology: Status and Roadmap of Space Agencies. Microbes and Environments. 2014; 29(3): 239-242. DOI: 10.1264/jsme2.ME2903rh.
Venkateswaran K, La Duc MT, Horneck G. Microbial existence in controlled habitats and their resistance to space conditions. Microbes and Environments. 2014 September 17; 29(3): 243-249. DOI: 10.1264/jsme2.ME14032. PMID: 25130881.
Ichijo T, Hieda H, Ishihara R, Yamaguchi N, Nasu M. Bacterial monitoring with adhesive sheet in the International Space Station-"Kibo", the Japanese experiment module. Microbes and Environments. 2013 April 20; 28(2): 264-268. DOI: 10.1264/jsme2.ME12184. PMID: 23603802.
Makimura K, Satoh K, Sugita T, Yamazaki TQ. Fungal Biota in Manned Space Environment and Impact on Human Health. Nippon Eiseigaku Zasshi. 2011; 66(1): 77-82. DOI: 10.1265/jjh.66.77. PMID: 21358138. [Japanese]
Yamaguchi N, Roberts MS, Castro S, Oubre C, Makimura K, Leys N, Grohmann E, Sugita T, Ichijo T, Nasu M. Microbial monitoring of crewed habitats in space—Current status and future perspectives. Microbes and Environments. 2014; 29(3): 250-260. DOI: 10.1264/jsme2.ME14031. PMID: 25130885.
Satoh K, Yamazaki TQ, Nakayama T, Umeda Y, Alshahni MM, Makimura M, Makimura K. Characterization of fungi isolated from the equipment used in the International Space Station or Space Shuttle. Microbiology and Immunology. 2016 May; 60(5): 295-302. DOI: 10.1111/1348-0421.12375. PMID: 26969809.
Ground Based Results Publications
Kawaguchi Y, Yang Y, Kawashiri N, Shiraishi K, Takasu M, Narumi I, Satoh K, Hashimoto H, Nakagawa K, Tanigawa Y, Momoki Y, Tanabe M, Sugino T, Takahashi Y, Shimizu Y, Yoshida S, Kobayashi K, Yokobori S, Yamagishi A. The possible interplanetary transfer of microbes: Assessing the viability of deinococcus spp. under the ISS environmental conditions for performing exposure experiments of microbes in the Tanpopo Mission. Origins of life and evolution of the biosphere: The Journal of the International Society for the Study of the Origin of Life. 2013 October 15; 43(4): 411-428. DOI: 10.1007/s11084-013-9346-1. PMID: 24132659. [Also: Paper presented at the 12th European Workshop on Astrobiology “EANA’12” in Stockholm, Sweden. (October 15 to 17, 2012). Editors Axel Brandenburg and Nils Holm.]