Influencing Factors of Space Flight on Expression of Strains of Interleukin-1 (ARIL-1) - 10.25.17

Overview | Description | Applications | Operations | Results | Publications | Imagery

ISS Science for Everyone

Science Objectives for Everyone
Aryl (Aril) will develop methods for controlled changing of the properties of recombinant producer strains of interleukins 1alpha, 1beta and aryl through exposure of germ cultures to orbital flight environments with subsequent selection in laboratory conditions.
Science Results for Everyone
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The following content was provided by L. N. Petrov, G. Y. Scherbakov, M.D., Ye N. Sventitski, and is maintained in a database by the ISS Program Science Office.
Experiment Details


Principal Investigator(s)
L. N. Petrov, Biopreparat, Russia
G. Y. Scherbakov, M.D., Biopreparat, Russia
Ye N. Sventitski, Russia

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Sponsoring Space Agency
Russian Federal Space Agency (Roscosmos)

Sponsoring Organization
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Research Benefits
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ISS Expedition Duration
October 2007 - September 2010

Expeditions Assigned

Previous Missions
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Experiment Description

Research Overview
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The experiment consists in determining the effects on the culture samples of the microgravity environment, movement in the geomagnetic field and exposure to space radiation. As a result, it is planned to find the optimal exposure conditions for recombinant microorganism cultures, which will assure the maximum copying ability of plasmids controlling the synthesis of interleukines and aryl, as well as develop methods for controlled changes in the properties of recombinant producer strains of interleukins 1alpha, 1beta, aryl.

To optimize exposure conditions for germ cultures of recombinant producer strains of interleukins 1alpha, 1beta and aryl to space flight environments. Obtaining recombinant strains of producers of bioactive substances with improved properties.

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Space Applications
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Earth Applications
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Operational Requirements and Protocols
Returned to Earth are Bioekologia cases and photographic materials taken during the experiment

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Decadal Survey Recommendations

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Results/More Information

During the Aryl-1, the culture retained the capability to synthesize recombinant protein (Aryl). Quantitatively speaking, the following differences were observed: when IPTG was used as the inducer, the amount of protein in the space experiment was lower than in ground conditions, while the opposite picture was observed when lactose was used. The maximum yield of Escherichia coli TGl (pPR-TGATG-hIL-1rα) biomass observed in microgravity conditions with induction by lactose was higher than in ground conditions. The result obtained can demonstrate the positive effect of microgravity on culture growth and on the high sensitivity of the culture to the nature of the inducer in these conditions.

In samples with lactose induction under microgravity conditions, a significant drop in the number of viable cells (CFU) was observed, which could be caused by both spaceflight factors and the oversaturation of cells by the target protein. This finding was confirmed in microscope observations and electrophoretic analysis.

The amount obtained in the space experiment of medication containing various forms of recombinant protein did not differ from that obtained under ground conditions. In the conditions of this experiment, most indicative were the initial cultivation doses of 107 and 109 cells/ml, at which the lowest degree of plasmid elimination was observed, and thus better synthesis of target protein by the cells of these cultures both on the ISS and on the ground.

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Related Websites
Energia - Science Research on the ISS Russian Segment

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