Effect of Spaceflight Factors on the Expression of Producer Strains of Interleukin 1α, 1β, and ARIL (ARIL-1) - 03.07.18

Overview | Description | Applications | Operations | Results | Publications | Imagery

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Science Objectives for Everyone
Effect of Spaceflight Factors on the Expression of Producer Strains of Interleukin 1α, 1β, and ARIL (ARIL) investigation develops a method of increasing the productivity of recombinant producer strains of interleukins 1a, 1b, and ARIL (interleukin antagonist) medication by incubating microorganism cultures in microgravity conditions and subsequently selecting the best cultures. The essence of the research is to identify possible changes in physical, chemical, morphological, and genetic properties of therapeutic and diagnostic bacteriophages exposed to spaceflight factors that could be used to obtain highly effective medicines with specific properties.
Science Results for Everyone
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The following content was provided by L. N. Petrov, G. Y. Scherbakov, M.D., Ye N. Sventitski, and is maintained in a database by the ISS Program Science Office.
Experiment Details

OpNom:

Principal Investigator(s)
L. N. Petrov, Biopreparat, Russia
G. Y. Scherbakov, M.D., Biopreparat, Russia
Ye N. Sventitski, Russia

Co-Investigator(s)/Collaborator(s)
Information Pending

Developer(s)
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Sponsoring Space Agency
Russian Federal Space Agency (Roscosmos)

Sponsoring Organization
Information Pending

Research Benefits
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ISS Expedition Duration
October 2007 - September 2010

Expeditions Assigned
16,17,18,19/20,21/22,23/24

Previous Missions
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Experiment Description

Research Overview
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Description
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Applications

Space Applications
The essence of the research is the identification of possible changes in the physical, chemical, morphological, and genetic properties of therapeutic and diagnostic bacteriophages exposed to spaceflight factors that will be used to obtain highly effective medicines with specific properties.

Earth Applications
The material obtained is used for selecting producer strains with enhanced properties (increased productivity and segregation stability) on Earth and subsequently introducing them into production. The selected high-productivity lines (clones) of producer strains of interferon, interleukins, and ARIL may be used in the production of biological medications at the State Scientific Research Institute of High Purity Biopharmaceuticals. The use in production of these strain medications with enhanced properties could have a significant economic impact

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Operations

Operational Requirements and Protocols
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Decadal Survey Recommendations

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Results/More Information

During the Aryl-1, the culture retained the capability to synthesize recombinant protein (Aryl). Quantitatively speaking, the following differences were observed: when IPTG was used as the inducer, the amount of protein in the space experiment was lower than in ground conditions, while the opposite picture was observed when lactose was used. The maximum yield of Escherichia coli TGl (pPR-TGATG-hIL-1rα) biomass observed in microgravity conditions with induction by lactose was higher than in ground conditions. The result obtained can demonstrate the positive effect of microgravity on culture growth and on the high sensitivity of the culture to the nature of the inducer in these conditions.

In samples with lactose induction under microgravity conditions, a significant drop in the number of viable cells (CFU) was observed, which could be caused by both spaceflight factors and the oversaturation of cells by the target protein. This finding was confirmed in microscope observations and electrophoretic analysis.

The amount obtained in the space experiment of medication containing various forms of recombinant protein did not differ from that obtained under ground conditions. In the conditions of this experiment, most indicative were the initial cultivation doses of 107 and 109 cells/ml, at which the lowest degree of plasmid elimination was observed, and thus better synthesis of target protein by the cells of these cultures both on the ISS and on the ground.

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Related Websites
Energia - Science Research on the ISS Russian Segment

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Imagery