Extraction and Investigation of surface glycoproteins E1-E2 Alphavirises on Earth and in Space (Glykoproteid-K) - 03.14.18

Overview | Description | Applications | Operations | Results | Publications | Imagery

ISS Science for Everyone

Science Objectives for Everyone
The Identifying and Investigation Surface Glycoproteins E1-E2 of Alphaviruses on Earth and in Space (Glikoproteid) obtains data on the atomic structure of glycoprotein biomolecules with the highest possible resolution to create effective and safe new generation vaccines and to develop new antiviral medications and diagnostic systems.
Science Results for Everyone
Information Pending

The following content was provided by K. A. Miroshnikov, G. Y. Scherbakov, M.D., and is maintained in a database by the ISS Program Science Office.
Experiment Details

OpNom:

Principal Investigator(s)
K. A. Miroshnikov, Shubnikiv Institut of Crystallograhy of Russian Academy of Sciences (IC RAS), Russia
G. Y. Scherbakov, M.D., Biopreparat, Russia

Co-Investigator(s)/Collaborator(s)
Information Pending

Developer(s)
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Sponsoring Space Agency
Russian Federal Space Agency (Roscosmos)

Sponsoring Organization
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Research Benefits
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ISS Expedition Duration
April 2005 - October 2005; April 2006 - September 2006; October 2007 - October 2008

Expeditions Assigned
11,13,16,17

Previous Missions
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Experiment Description

Research Overview
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Description
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Applications

Space Applications
The space grown crystals will be used to determine the 3-dimensional structure of the protein. This will facilitate the continuation of the refinement of the technology for obtaining bio-crystals in microgravity and the development of crystallographic research in space in order to obtain high-quality protein crystals.

Earth Applications
The surface glycoprotein Е of the West Nile virus is the main target for virus neutralizing antibodies. The accurate spatial mapping of neutralizing virus epitopes is of fundamental importance for the development of antiviral medications, vaccines, and diagnostic tests. The project implementation provided fundamentally new information on the structure of a new type of viral proteins and combined the accumulated information on the organization of the virions of an entire family of warm-blooded animal viruses.

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Operations

Operational Requirements and Protocols
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Decadal Survey Recommendations

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Results/More Information

As a result of the Glikoproteid experiment, in most cases, the presence of small prism-shaped crystals was identified with a maximum size of approximately, 100 micron in the presence of amorphous deposits. The development relates to the following aspects. First, to the principal role of the Е1 and Е2 glycoproteins in the development of the infection process and the generation of an immune response to an alphavirus infection. Second, to the prevalence of alphavirus infections among pets and humans. Third, to the structural specifics of viral proteins, which as a rule, are typical for a group of viruses and usually differ greatly from other proteins.

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Results Publications

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Ground Based Results Publications

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ISS Patents

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Related Publications

    Tsygannik IN, Artemyev IV, Arkhipova SF, Pletnyev VZ, Scherbakov GY.  Crystallization of proteins in microgravity  . Scientific and Technical Conference on the Main Results of Applied Scientific Research on the ISS RS, Korolev, Russia; 2007

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Related Websites
Energia - Science Research on the ISS Russian Segment

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Imagery

image Kriogem-03M Refridgerator maintains proper temperature onboard. Reusable heat-insulated container with cold accumulator TVK-M maintains proper temperature while returning the crystals to the Earth. CPCF Module grows the protein crystals in the orbit and delivery to the Earth. Activation Device activates crystal growth process.
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