Optimizing Heterologous Expression in Saccharomyces Yeast in Microgravity Based on the Example of Hepatitis B Surface Antigen Synthesis (Antigen (Antigene)) - 03.07.18

Overview | Description | Applications | Operations | Results | Publications | Imagery

ISS Science for Everyone

Science Objectives for Everyone
The Optimizing Heterologous Expression in Saccharomyces Yeast in Microgravity based on the Example of Hepatitis B Surface Antigen Synthesis (Antigen) investigation comparatively studies the specifics of the heterologous expression of the HbsAg gene of the Hepatitis B virus in the yeast Saccharomyces cerevisiae in microgravity and Earth. This investigation establishes methods for optimizing synthesis in order to obtain high-quality strains of producer yeasts of the Hbs antigen to create an effective vaccine for Hepatitis B.
Science Results for Everyone
Attack of the antigen clones! The surface antigen of the hepatitis B virus (HBsAg) indicates an active infection. This investigation sought to identify as many characteristics as possible of HBsAg in yeast and to compare those characteristics in microgravity and normal gravity. This could help scientists synthesize the antigen and produce clones with deviations in the antigen that could be passed on to offspring. The data confirmed the feasibility of breeding super-producers of the antigen, and showed that stability of HBsAg characteristics depends mainly on strain properties and experiment design and not on storage time on Earth or in space.

The following content was provided by L. A. Nesterova, and is maintained in a database by the ISS Program Science Office.
Experiment Details

OpNom:

Principal Investigator(s)
L. A. Nesterova, Biopreparat, Russia

Co-Investigator(s)/Collaborator(s)
Information Pending

Developer(s)
Information Pending

Sponsoring Space Agency
Russian Federal Space Agency (Roscosmos)

Sponsoring Organization
Information Pending

Research Benefits
Information Pending

ISS Expedition Duration
April 2006 - April 2009

Expeditions Assigned
13,14,15,16,17,18

Previous Missions
Information Pending

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Experiment Description

Research Overview
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Description
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Applications

Space Applications
The results obtained demonstrate the possibility of the suitable existence of microorganisms aboard spacecraft as a part of biotechnological factories that in the future will accompany extended interplanetary flights. The space investigations demonstrated that microorganism cultures with the needed properties can be selected both on Earth and in space.

Earth Applications
Studying the specifics and factors of HBs-antigen gene expression stability in yeast cells is relevant to optimize the large-scale production of a yeast recombinant vaccine for Hepatitis B.

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Operations

Operational Requirements and Protocols
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Decadal Survey Recommendations

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Results/More Information

An analysis of the data obtained confirmed the feasibility of applying new methods to line breeding, super-producers of the HBs-antigen. Based on the data obtained, the following conclusions can be drawn: the stability of HBs-antigen characteristics does not depend on storage time on Earth or in space conditions, and it is mainly determined by strain properties and experiment design conditions.

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Related Websites
Energia - Science Research on the ISS Russian Segment

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Imagery