Effect of Microgravity on Kinetic Properties of Enzymtic Reactions - Hydrolysis of Sucrose in Cerevisiae Cells of Saccharomycetes (SMEK) - 11.22.16

Overview | Description | Applications | Operations | Results | Publications | Imagery

ISS Science for Everyone

Science Objectives for Everyone
MEK (Effects of Micro-g on Fermentative Kinetics) is a study of the kinetics (rates) of enzymatic reactions with lipase and invertase (these break down fats and sugars respectively).
Science Results for Everyone
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The following content was provided by S. Romanov, and is maintained in a database by the ISS Program Science Office.
Experiment Details


Principal Investigator(s)
S. Romanov, RSC Energia, Russia

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Sponsoring Space Agency
Russian Federal Space Agency (Roscosmos)

Sponsoring Organization
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Research Benefits
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ISS Expedition Duration
April 2006 - September 2006

Expeditions Assigned

Previous Missions
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Experiment Description

Research Overview
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To study the effect of microgravity on kinetic parameters of enzymatic reactions. To study differences in the hydrolysis of sucrose in cerevisiae cells of saccharomycetes.

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Space Applications
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Earth Applications
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Operational Requirements and Protocols
Measurement of the enzymatic reaction activity by the example of three enzymes (invertase, lipase and immobilized lipase).
In design experimental equipment is implemented as a unit consisting of two parts: Unit of MEK chambers and MEK electronic board connected by a cable. The unit of MEK chambers houses three blocks isolated from each other and filled with experimental enzymes. Each experimental block accommodates 5 chambers with enzymes. The first experimental block houses 5 chambers with lipase.

The second block accommodates 5 chambers with immobilized (in silicon) lipase. The third block contains 5 chambers with invertase (of baker's yeast). Each ampoule is filled with olive oil of different concentration in water emulsion of polyvinyl alcohol in the range from 100 to 500 millimoles. The MEK electronic board is a control unit as a plug-in system board on which all information on the experiment progress is recorded.

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Decadal Survey Recommendations

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Results/More Information

Exposed samples of three enzymes (invertase, lipase and immobilized lipase) in microgravity conditions and information recorded on the system board.

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Related Websites
Energia - Science Research on the ISS Russian Segment

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image The MEK experimental equipment includes, unit of MEK chambers (with a set of cables).
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