Influence of the Flow of Heavily Charged Particles of Space Radiation on Gentic Properties of Cell Production (Biotrack-A) - 10.25.17

Overview | Description | Applications | Operations | Results | Publications | Imagery

ISS Science for Everyone

Science Objectives for Everyone
Biotrack-A focused on the effects of cosmic radiation on recombination among bacterial strains.
Science Results for Everyone
Information Pending

The following content was provided by O. E. Krasheninnikova, Tatiana K. Krasheninnikova, and is maintained in a database by the ISS Program Science Office.
Experiment Details

OpNom:

Principal Investigator(s)
O. E. Krasheninnikova, Russia
Tatiana K. Krasheninnikova, Institute for Applied Biochemistry and Machine Building (Biohimmash), Russia

Co-Investigator(s)/Collaborator(s)
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Developer(s)
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Sponsoring Space Agency
Russian Federal Space Agency (Roscosmos)

Sponsoring Organization
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Research Benefits
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ISS Expedition Duration
April 2006 - March 2013

Expeditions Assigned
13,14,15,16,17,18,19/20,21/22,23/24,25/26,27/28,29/30,31/32,33/34

Previous Missions
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Experiment Description

Research Overview
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Description
Study a correlation between the alteration of genetic properties, productivity of recombinant strains in microgravity, and exposure to heavy charged space particles.

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Applications

Space Applications
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Earth Applications
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Operations

Operational Requirements and Protocols
Vial with an ampoule with lyophilized cultures, Tube with biosample vials, Temperature data logger, Thermoluminescent detector, Track detector
Study a long-duration effect of space radiation factors on cultures of recombinant strains producing biologically active substances in spaceflight, as well as their growth characteristics and plasmid segregation stability. Study and test a technique for selecting highly productive strains from the cultures exposed in spaceflight. Record and analyze heavy nuclear tracks and total radiation dose. Determine a correlation between alterations of genetic properties of the subject biological objects with a different level of organization (microorganisms, plant and animal cells) and number of hits of heavy charged space particles.

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Decadal Survey Recommendations

Information Pending

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Results/More Information

As a result of the Biotrek space experiment, four Bioekolgiya kits (4 cases per kit) were successfully delivered and all 16 were successfully returned. The space experiment was conducted from 93-2069 days, starting in ISS-14 and ending in ISS-34. Preserving the viability of the bacterial cultures on the International Space Station Russian Segment (ISS RS) to a great degree depended on the culture strain: if the museum strain in Earth conditions has a tendency for the cell concentration to decrease during storage, then the trend was maintained as a result of being on the ISS RS.

According to the macro-morphological assessment, the most stable properties were maintained in the museum strains and cultures exposed in vials. For cultures located throughout the entire experiment in solid and liquid nutrient media, a typical manifestation was a large number of different isolates in S (virulent strains) and R (non-virulent colonies) form. The number of dissociated forms within one type increased with experiment time, and also depended on the microorganism itself. Cultures in the space experiment which were in suspended form manifested polymorphism. The formation in a suspended culture of cells of varying lengths and varying stages of growth depended on the combination of the conditions in the culture. Culture productivity when cultured in liquid nutrient medium was at the level of standard laboratory indicators. The decrease in cell culture concentration in vials and in liquid nutrient medium after the end of the experiment did not have an impact on productivity; cultures fully recovered their properties upon multiple passaging to nutrient media in laboratory conditions. While studying the dissociated forms of cultures, it was shown that cultures stably retain the potential for active growth, and high cell concentrations were obtained when it is originally programmed in the genes of the cell by nature. All these parameters did not depend on the time a strain spent on the ISS RS and were comparable to ground controls.

For the first time in Russia, after the longest exposure time in the Biotrek experiment over 2069 days, results were obtained for bacterial culture strains of Arthrobacter sp. OC-1, Arthrobacter sp. МИА-74, Arthrobacter sp. МИП-89, Bacillus Licheniformis L-34, and of micromycetes Cylindrocarpon radicicola Wollenweber НТН-10, Mycelium sterilia ЛХ-1, Mycelium radicis ginseng НТН-1, and Mycelium radicis var. ledum (Phialocephala fortini) НЖ-13, demonstrating that these lyophilized bacterial and fungal cultures survived in solid nutrient medium and retained the main performance indicators. Also for the first time, a molecular and genetic comparison was conducted of in-flight, Earth, and collection samples of cultures of bacteria Arthrobacter sp. OC-1 and micromycetes Mycelium radicis var. ledum (Phialocephala fortini) НЖ-13. The comparative analysis of the versions of bacterial culture strains identifies genetic changes in the flight versions of the strains by fingerprinting demonstrated genomic changes in the culture strain Arthrobacter sp. OC-1 in the form of single-point replacements of individual nucleotides and slight deletion or inversion occurring both in Earth conditions and in space. New bacterial and fungal strains were obtained with enhanced growth and biosynthesis properties from the Biotrek experiment. All the bacterial cultures in the Biotrek experiment were lyophilized and added to the culture collection bank at the space biotechnology laboratory at ОАО Biokhimmash.

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Related Websites
Energia - Science Research on the ISS Russian Segment

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Imagery