Multi-Omics Analysis of Human Microbial-Metabolic Cross-talk in the Space Ecosystem (Multi-Omics-Mouse) - 05.30.18

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The Multi-Omics Analysis of Human Microbial-Metabolic Cross-talk in the Space Ecosystem (Multi-Omics-Mouse) investigation evaluates the impact of the space environment and prebiotics on mice immune function. This is accomplished by combining the data obtained from the measurements of changes in the gut microbiological composition, metabolites profiles, and the immune system in the subject mice.
Science Results for Everyone
Information Pending

The following content was provided by Natsuhiko Inoue, Ph.D., and is maintained in a database by the ISS Program Science Office.
Information provided courtesy of the Japan Aerospace and Exploration Agency (JAXA).
Experiment Details

OpNom: Multi-Omics-Mouse

Principal Investigator(s)
Hiroshi Ohno, M.D., Ph.D., RIKEN Center for Integrative Medical Science, Yokohama, Japan

Masahira Hattori, Ph.D., Department of Computational Biology, Center for Omics and Bioinformatics (Director), Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Japan
Jun Kikuchi, Ph.D., RIKEN Center for Sustainable Resource Science, Yokohama, Japan
Toshiko Ohta, Ph.D., Kagawa Nutrition University, Sakado, Japan
Masaharu Kagawa, Ph.D., Kagawa Nutrition University, Sakado, Japan
Tamotsu Kato, Ph.D., JAXA Space Biomedical Research Office, Tsukuba, Japan
Brian E. Crucian, Ph.D., NASA Johnson Space Center, Houston, TX, United States

Tsukuba Space Center, Tsukuba, Ibaraki, Japan

Sponsoring Space Agency
Japan Aerospace Exploration Agency (JAXA)

Sponsoring Organization
Japan Aerospace Exploration Agency

Research Benefits
Space Exploration, Earth Benefits, Scientific Discovery

ISS Expedition Duration
September 2016 - February 2018

Expeditions Assigned

Previous Missions
Information Pending

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Experiment Description

Research Overview
It has been suggested that living aboard the International Space Station (ISS) likely causes immune dysfunction in crew members, but the precise underlying mechanisms for this dysfunction is not well understood. Recent studies indicate that imbalance in gut microbiota composition, or dysbiosis, resulting from a variety of environmental stresses, could lead to various diseases including immune system dysfunction. Therefore, metagenomic analysis of the gut microbiota is important. Data from the observation of mice living on-orbit can complement astronauts’ data by presenting tissue samples that cannot be collected in human research. By combining mouse and human research results, candidates of bacterial and/or metabolic biomarkers for immune dysfunction can be identified, which could then be utilized for the development methods to amend observed microbial imbalances in humans during spaceflight.

Information Pending

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Space Applications
Multi-Omics-Mouse supports space exploration by expanding understanding of immune system function in space. A better understanding of how digestive tract microbial communities affect immune organs can provide information in the development of strategies for supporting astronaut health during long-term space missions.

Earth Applications
Multi-Omics-Mouse provides new information on the microbial ecology of the mammalian digestive tract under the unusual conditions of spaceflight. This information shows the growing field of microbiology, and can strengthen understanding of the links between overall health and digestive tract flora.

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Operational Requirements and Protocols
Twelve mice, consisting of 6 mice normal-food-fed and the others 5% fructo-oligosaccharide (FOS)-fed live in microgravity, or artificial gravity, aboard the International Space Station (ISS) for 4 weeks. Fecal samples are collected pre-flight, in-flight (L+2 weeks, L+4 weeks), and post-flight. After return to Earth, blood, and various tissues including thymus, spleen, small intestine, Peyer's patch, large intestine, blind gut content, inguinal lymph, adrenal gland, bone marrow, liver, and muscle are also collected. Ground control mice are treated as same as space-flown mice. These samples are subjected to metagenome, metabolome, gene expression profiling analysis in order to reveal the whole picture of changes in intestinal microbiota in response to space/ground environmental differences.

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Decadal Survey Recommendations

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Results/More Information

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Related Websites

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