NanoRacks-Valley Christian High School-Bacterial Growth and Kanamycin Resistance in a Microgravity Environment (NanoRacks-VCHS-Bacterial Growth and Kanamycin Resistance) - 09.27.17

Overview | Description | Applications | Operations | Results | Publications | Imagery

ISS Science for Everyone

Science Objectives for Everyone
In microgravity, some types of bacteria can grow more rapidly and with greater strength than they do on Earth. Current and future space missions rely on antibiotics to treat and prevent diseases caused by bacteria. NanoRacks-Valley Christian High School-Bacterial Growth and Kanamycin Resistance in a Microgravity Environment (NanoRacks-VCHS-Bacterial Growth and Kanamycin Resistance) compares how well Escherichia coli (E. coli) samples grow, and whether the antibiotic Kanamycin kills them more effectively, in microgravity and on Earth.
 
Science Results for Everyone
Information Pending

The following content was provided by Angie Chen, and is maintained in a database by the ISS Program Science Office.
Experiment Details

OpNom: NanoRacks Module-16 S/N 1002

Principal Investigator(s)
Valley Christian High School , Valley Christian High School, San Jose, CA, United States

Co-Investigator(s)/Collaborator(s)
Dan Saldana, Valley Christian High School, San Jose, CA, United States

Developer(s)
Valley Christian High School , San Jose , CA, United States
NanoRacks, LLC, Webster, TX, United States

Sponsoring Space Agency
National Aeronautics and Space Administration (NASA)

Sponsoring Organization
National Laboratory Education (NLE)

Research Benefits
Scientific Discovery

ISS Expedition Duration
March 2014 - September 2014

Expeditions Assigned
39/40

Previous Missions
Information Pending

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Experiment Description

Research Overview

  • NanoRacks-Valley Christian High School-Bacterial Growth and Kanamycin Resistance in a Microgravity Environment (NanoRacks-VCHS-Bacterial Growth and Kanamycin Resistance) is needed to determine how microgravity affects bacteria and antibiotics in spacecraft and potential future settlements.
     

  • The research can be applied in treating astronauts’ bacterial infections.
     

Description

NanoRacks-Valley Christian High School-Bacterial Growth and Kanamycin Resistance in a Microgravity Environment (NanoRacks-VCHS-Bacterial Growth and Kanamycin Resistance) grows a sample of Escherichia coli (E. coli) K-12 using lysogeny broth (LB), by pumping the broth into the bacterial chamber, which contains lyophilized (freeze-dried) E. coli K-12. After four days of growth, kanamycin (antibiotic) is introduced to the sample in order to kill the bacteria. Photos are taken every hour (increased to every 15 minutes during the growth phase). Green fluorescent protein (GFP) fluorescence acts as the growth indicator, glowing green when E. coli is alive, and Propidium iodide acts as the death indicator, turning red when the bacterial cells lyse.
 

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Applications

Space Applications
The investigation studies the behavior of both bacteria and antibacterial drugs in microgravity, providing new insight that benefits crewmembers' health. Bacteria samples are grown for four days and then exposed to Kanamycin, which is designed to kill them. Photographs every hour show how much bacteria is still growing after exposure to the antibiotic, and results are compared with ground samples.
 

Earth Applications
Students designed and built the investigation, learning about bacterial growth, lab equipment and safety, and hardware design. Results may lead to new types of medicine to treat bacterial infections, including bacteria that have evolved to resist common antibiotics.
 

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Operations

Operational Requirements and Protocols

NanoRacks Module-16 is completely autonomous and only requires installation and removal. During operation, photographic data is sent to investigators to track experiment progress. The first 3 days have the most data transmitted (about 16 VGA quality photos along with environmental data (humidity and temperature). Thereafter, transmission is limited to 1 VGA photo and environmental data per day for the remainder of flight. The payload chamber needs to be returned to the researchers so its contents can be examined under an atomic force microscope.


Crew involvement with NanoRacks Module-16 is limited to transferring the NanoRacks locker insert from the launch vehicle to the ISS, installation and activation of the NanoRacks Frames into the EXPRESS rack locker, cleaning of the air inlet filter (as necessary), and data retrieval (as needed) during the mission.

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Decadal Survey Recommendations

Information Pending

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Results/More Information

Information Pending

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Related Websites

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Imagery

image

The NanoRacks-Valley Christian High School-Bacterial Growth and Kanamycin Resistance in a Microgravity Environment (NanoRacks-VCHS-Bacterial Growth and Kanamycin Resistance) investigation team.  Image courtesy of Valley Christian High School.


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