Magnetic 3D Cell Culture for Biological Research in Microgravity (Magnetic 3D Cell Culturing) - 04.26.17
Cell cultures in space spontaneously grow in three dimensions (3D), which results in characteristics more representative of how cells grow and function in living organisms. But in microgravity, routine manipulation of cell cultures is challenging. Magnetic 3D Cell Culture for Biological Research in Microgravity (Magnetic 3D Cell Culturing) uses magnetized cells and tools to make it easier to handle cells and cultures, and to improve the reproducibility of experiments. This approach also makes it possible to generate two-dimensional (2D) cultures as controls, and to determine whether biological events in these monolayer cultures result from gravity or substrate attachment. Science Results for Everyone
Information Pending Experiment Details
OpNom: Magnetic 3D Cultures
Glauco Souza, Ph.D., n3D Biosciences, Inc., Houston, TX, United States
Jeanne L. Becker, Ph.D., Astrogenetix, Austin, TX, United States
Luis Zea, Ph.D., BioServe Space Technologies, University of Colorado, Boulder, Boulder, CO, United States
Stefanie Countryman, M.B.A., BioServe Space Technologies, Boulder, CO, United States
Louis S. Stodieck, Ph.D., University of Colorado, BioServe Space Technologies, Boulder, CO, United States
BioServe Space Technologies, University of Colorado, Boulder, CO, United States
Sponsoring Space Agency
National Aeronautics and Space Administration (NASA)
National Laboratory (NL)
ISS Expedition Duration
April 2017 - September 2017
- Magnetic 3D Cell Culture for Biological Research in Microgravity (Magnetic 3D cell culturing) incorporates magnetic cell culture technology into existing flight hardware to develop optimal operating conditions to support continued cell culturing activities on the International Space Station (ISS).
- Magnetic 3D Cell Culturing establishes magnetic bioprinting as a platform for two-dimensional (2D) and three-dimensional (3D) cell culture on the ISS.
- Magnetic 3D Cell Culturing seeks to overcome the practical challenge of media exchange without losing cells in culture, and of handling and retrieving cell cultures for analysis.
- Magnetic 3D Cell Culturing enables engineering of complex tissue structures that exhibit relevant tissue-like spatial architecture.
- Magnetic 3D Cell Culturing enables the ability to perform 2D cell culture to provide much needed controls for space-based cell culture research and comparisons with ground studies.
- Magnetic 3D Cell Culturing improves throughput and scalability of experiments at the ISS.
Magnetic tools integrated with existing flight-certified hardware add a platform for cell culture research in space. This platform provides a way to manipulate and culture cells in 2D and 3D in space and on the ground, which can help isolate the effects of gravity in experiments and enable biological research previously deemed unfeasible in space.
Validation of magnetic cells and tools for growth of 3D cultures in microgravity has value for research on Earth. A growing demand exists for in vitro or culture models that better capture the characteristics of tissue in living organisms. The capability to recreate such environments on Earth almost as easily as in space could potentially reduce drug development costs.
Operational Requirements and Protocols
Decadal Survey Recommendations
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Nano3D Biosciences, Inc.
Nature, “Cell culture: a better brew”, April 2013
Nature Medicine, “Startups tout commercially 3D-printed tissue for drug screening”, January 2015
NBC News, “Magnetic Levitation Grows Realistic Lung Tissue”, February 2013
Popular Science, “Researchers make super-realistic artificial lung tissue by levitating cells”, 2013
MIT Technology Review, “Bio-Assembling in 3-D with Magnetic Levitation, A New System Grows Tissue in 3-D without Protein Matrixes”, 2011
TEDx Houston 2012 RESONATE