DNA Photodamage:  Measurements of Vacuum Solar Radiation-induced DNA Damages within Spores (EXPOSE-R PHOTO) - 11.22.16

Overview | Description | Applications | Operations | Results | Publications | Imagery

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The DNA Photodamage:  Measurements of Vacuum Solar Radiation-induced DNA Damages within Spores (EXPOSE-R PHOTO) experiment studies the effect of exposure of bacterial spores, and samples of their DNA, to solar UV radiation. The EXPOSE programme is part of ESA’s research in astrobiology, i.e. the study of the origin, evolution and distribution of life in the universe. EXPOSE offers one to two years of exposure with full access to all components of the harsh space environment: cosmic radiation, vacuum, full-spectrum solar light including UV-C, freezing/thawing cycles, microgravity.
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Information Pending

The following content was provided by Hervé Cottin, and is maintained in a database by the ISS Program Science Office.
Information provided courtesy of the Erasmus Experiment Archive.
Experiment Details

OpNom:

Principal Investigator(s)
Jean Cadet, SCIB-UMR-E n°3 (CEA/UJF) Institut Nanosciences et Cryogénie CEA/ Grenoble , Grenoble, France

Co-Investigator(s)/Collaborator(s)
Thierry Douki, SCIB-UMR-E n°3 (CEA/UJF) Institut Nanosciences et Cryogénie CEA/ Grenoble , Grenoble, France
J. L. Ravanat, France
S. Sauvaigo, France

Developer(s)
European Space Agency (ESA), Noordwijk, Netherlands

Sponsoring Space Agency
European Space Agency (ESA)

Sponsoring Organization
Information Pending

Research Benefits
Information Pending

ISS Expedition Duration
October 2008 - March 2011

Expeditions Assigned
18,19/20,21/22,23/24,25/26

Previous Missions
Information Pending

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Experiment Description

Research Overview

Some EXPOSE experiments investigate to what extent particular terrestrial organisms are able to cope with extra-terrestrial environmental conditions. Other EXPOSE experiments test how organic molecules, the building blocks of life, behave when subjected for a prolonged period of time to unfiltered solar light (a scientific domain called chemical evolution).
 
DNA Photodamage:  Measurements of Vacuum Solar Radiation-induced DNA Damages within Spores (EXPOSE-R PHOTO) studies the effect of exposure of bacterial spores of Bacillus subtilis, and samples of their DNA, to solar UV radiation. The objective is to assess the quantity and chemistry of chemical products produced. The samples are completely exposed, or protected by artificial meteorite materials, clays, and salt crystals.

Description
Information Pending

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Applications

Space Applications
Expose-R contains a variety of biological samples including plant seeds, bacteria, fungi and ferns, which are exposed to the harsh space environment for 22 months, to gain a better understanding of how living things react to the conditions.

Earth Applications
Information Pending

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Operations

Operational Requirements and Protocols

The photoproducts resulting from the exposure of bacterial spores of Bacillus subtilis to solar extraterrestrial UV radiation, organic compounds are exposed to the space environment. Reference samples are kept in darkness.
 
At the end of the 22 months exposure period the Expose-R trays are retrieved from their location outside the station and returned to Earth and distributed to scientists for further analysis.

Nine experiments contained in three trays make up Expose-R, which is mounted on the outside of the Russian segment of the International Space Station.
 
 

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Decadal Survey Recommendations

Information Pending

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Results/More Information

Information Pending

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Results Publications

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Ground Based Results Publications

    Rabbow E, Horneck G, Rettberg P, Schott J, Panitz C, L'Afflitto A, von Heise-Rotenburg R, Willnecker R, Baglioni P, Hatton JP, Dettmann J, Demets R, Reitz G.  EXPOSE, an Astrobiological Exposure Facility on the International Space Station - from Proposal to Flight. Origins of life and evolution of the biosphere: The Journal of the International Society for the Study of the Origin of Life. 2009; 39(6): 581-598. DOI: 10.1007/s11084-009-9173-6.

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ISS Patents

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Related Publications

    Cadet J, Douki T, Ravanat JL, Wagner JR.  Measurement of oxidatively generated base damage to nucleic acids in cells: facts and artifacts. Bioanalytical Reviews. 2012 December; 4(2-4): 55-74. DOI: 10.1007/s12566-012-0029-6.

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Related Websites
Expose-R PHOTO Information from ESA's Erasmus Experiment Archive

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Imagery

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NASA Image: ISS018E013542 - Yury Lonchakov in the Pirs Docking Compartment (DC1) as he works with an EXPOSE-R sample tray.

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NASA Image: ISS018E039227 - One of two Expedition 18 spacewalkers provided this close-up image of the Expose-R experiment, reinstalled a short while earlier on the outside of the Russian segment of the International Space Station. The European experiment is equipped with three trays which contain a variety of biological samples.

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NASA Image: ISS021E027095 - The Expose-R experiment located on the exterior of the Service Module (SM)/Zvezda.

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NASA Image: ISS025E015290 - Russian cosmonaut Oleg Skripochka, Expedition 25 flight engineer, wearing a Russian Orlan spacesuit, is photographed working near the ESA experiment EXPOSE-R instrument installed on the Zvezda Service module. Photo taken during the Russian EVA 26.

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NASA Image: ISS026e021253 - EXPOSE R - Russian Experiment, in the Service Module (SM). Photo was taken during Expedition 26.

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