Muscle Biopsy (MUSCLE BIOPSY) - 10.18.17

Overview | Description | Applications | Operations | Results | Publications | Imagery

ISS Science for Everyone

Science Objectives for Everyone
Skeletal muscle functions are mediated by cellular signalling mechanisms that may undergo considerable changes due to muscle activity or inactivity on Earth. Nitric oxide (NO) generated by NO-synthase (NOS) is a unique signalling molecule mediating basic physiological muscle functions, and NOS/NO signalling is linked to muscle activity in both humans and rodent animals. The first and major goal of this investigation is to collect, analyze, and interpret the data of NOS expression in fast and slow-type human skeletal muscle before spaceflight and following exposure to actual microgravity in space.
Science Results for Everyone
Information Pending

The following content was provided by Dieter Blottner, Ph.D., and is maintained in a database by the ISS Program Science Office.
Information provided courtesy of the Erasmus Experiment Archive.
Experiment Details

OpNom:

Principal Investigator(s)
Dieter Blottner, Ph.D., Charite Campus Benjamin Franklin, Berlin, Germany

Co-Investigator(s)/Collaborator(s)
Information Pending

Developer(s)
European Space Agency (ESA), Noordwijk, Netherlands

Sponsoring Space Agency
European Space Agency (ESA)

Sponsoring Organization
Information Pending

Research Benefits
Earth Benefits, Scientific Discovery

ISS Expedition Duration
September 2015 - March 2016; March 2016 - February 2018; -

Expeditions Assigned
45/46,47/48,49/50,51/52,53/54,57/58,59/60

Previous Missions
Information Pending

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Experiment Description

Research Overview
Skeletal muscle functions are mediated by cellular signalling mechanisms that may undergo considerable changes due to muscle activity or inactivity on Earth. Nitric oxide (NO) generated by NO-synthase (NOS) is a unique signalling molecule mediating basic physiological muscle functions including microperfusion capacity, soreness and muscle force generation capacity. NOS/NO signalling is linked to muscle activity in both humans and rodent animals, it can be restored together with other important signalling molecules (Homer) by resistive exercise / vibration in muscle and at the nerve-muscle interface (neuromuscular junction), and thus may serve as molecular biomarker for testing the effectiveness of countermeasure protocols to support human skeletal muscle performance capacity under real microgravity conditions. The first and major goal is to collect, analyze, and interpret the data of NOS expression in fast and slow-type human skeletal muscle before spaceflight (ground reference control) and following exposure to actual microgravity in space.

Description
Information Pending

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Applications

Space Applications
By improving the understanding of the mechanisms behind loss of muscle mass in space we in turn can develop more effective countermeasures for the crews, whether pharmacological, dietary or exercise-based in order to alleviate such adverse effects and hence improve/maintain the health and performance of our astronauts in orbit.

Earth Applications
By improving the understanding of the mechanisms behind loss of muscle mass in space and developing appropriate and effective countermeasures to any adverse effects we can also draw conclusions and get insights into certain muscular conditions on Earth. An adaptation of countermeasures originally developed for in-space use by astronauts, or newly developed ground-based countermeasures could be used within rehabilitation of patients affected by such medical conditions.

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Operations

Operational Requirements and Protocols
• Pre-flight muscle biopsy shall be taken less between L-120d and launch
• Post-flight muscle biopsy as soon as possible after landing i.e., 28 hour post-landing should be the latest time point.
• Immediate deep freezing of muscle biopsies (within 1-3 min.) in fl.N2 after sampling is strongly required.

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Decadal Survey Recommendations

Information Pending

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Results/More Information

Information Pending

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Related Websites

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Imagery