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NanoRacks Plate Reader (NanoRacks_Plate_Reader)
03.22.12
Scientists and payload developers can get more information on International Space Station research facilities by contacting the ISS Payloads Office or at 281-244-6187.

Overview | Description | Applications | Operations | Results | Publications | Images

Facility/Payload Overview

Brief Facility Summary

NanoRacks Plate Reader is a laboratory instrument designed to detect biological, chemical or physical events of samples in microtiter plates. Microplate readers are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations.

Facility Manager(s)

  • Michael Johnson, , NanoRacks, LLC, Houston, TX, United States

    • Co-Facility Manager(s)

    Information Pending

    Facility Developer

    NanoRacks, LLC, Houston, TX, United States

    Sponsoring Agency

    National Aeronautics and Space Administration (NASA)

    Expeditions Assigned

    29/30, 31/32

    Previous ISS Missions

    Information Pending

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    Facility/Payload Description

    Facility Summary

    • NanoRacks Plate Reader is a modified SpectraMax® M5e Multi-Mode Microplate Reader.


    • The SpectraMax® M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes.


    • NanoRacks Plate Reader possesses five modes, UV-Visible Absorbance, Fluorescence Intensity, Time-Resolved Fluorescence, Fluorescence Polarization, and Glow Luminescence, providing the benefit of multiple detection modes in one platform.

    Description

    NanoRacks Plate Reader is a modified SpectraMax® M5e Multi-Mode Microplate Reader. The SpectraMax® M5e Multi-Mode Microplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates.

    Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed optical pathlength cuvette. This temperature-independent normalization corrects for varying well volumes and can eliminate standard curves by allowing the calculation of concentrations directly from the absorbance with a known extinction coefficient, as exemplified with nucleic acid and protein quantitation.

    For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the optical design provides the highest level of flexibility. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Assays can be better optimized by scanning across a range of wavelengths in increments as small as 1 nm. Up to 4 wavelength pairs can be read in one protocol for endpoint and kinetic measurements.

    For luminescence, the NanoRacks Plate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays.

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    Operations

    Facility Operations

    Information Pending

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    Results/More Information

    Information Pending

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    Availability

    Information Pending

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    Related Web Sites
  • NanoRacks LLC
  • Molecular Devices

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    Publications

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    Related Publications

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    Images

    Information provided by the investigation team to the ISS Program Scientist's Office.
    If updates are needed to the summary please contact JSC-ISS-Program-Science-Group. For other general questions regarding space station research and technology, please feel free to call our help line at 281-244-6187 or e-mail at JSC-ISS-Payloads-Helpline.
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