NanoRacks-Valley Christian High School- Bacillus Subtilis Bacteria Growth (NanoRacks-VCHS-B. Subtilis) is a NanoLab project studying the growth and growth rate of Bacillus subtilis bacteria in microgravity.Principal Investigator(s)
NanoRacks, LLC, Houston, TX, United States
National Aeronautics and Space Administration (NASA)Sponsoring Organization
National Laboratory Education (NLE)ISS Expedition Duration
September 2011 - May 2012
29/30Previous ISS Missions
NanoRacks-Valley Christian High School-Bacillus Subtilis Bacteria Growth (NanoRacks-VCHS-B. Subtilis) examines the growth of Bacillus subtilis (B. subtilis) in microgravity. The bacteria are in dried stasis endospore mode (powder) in a bag, allowing the bacteria to survive in its dormant state until it reaches the International Space Station (ISS). Once aboard the ISS, the bacteria are invigorated by a nutrient enriched broth, which causes it to grow. The growth rate of the bacteria is determined by using two methods. First, using three light sensors, the amount of light from twelve LEDs that passed through the bacteria and broth mixture is measured. As more bacteria grow within the broth, less light is able to penetrate the bacterial broth. This is due to the accumulating bacteria eventually clouding the mixture. The second method utilized is to take a series of photos from a mounted camera within the Microlab. These pictures provide a qualitative depiction of the cloudy broth and bacterial colony.
In order to ensure crew health during space flight and long duration missions, an understanding of how bacteria behave in microgravity leads to better prevention and treatment of infections.Earth Applications
NanoRacks-VCHS-B. Subtilis allows students at Valley Christian High School to participate in an out of this world experiment to determine how microgravity affects the growth of bacteria. A better understanding of bacterial behavior on earth is accomplished by studying the fundamental properties of bacterial growth in microgravity.
NanoRacks Module-18 is completely autonomous and only requires installation and removal. NanoRacks Module–18 does not return to earth.Operational Protocols
Crew interaction with Module-18 is limited to transferring the NanoRacks locker Insert from the launch vehicle to the ISS, installation and activation of the NanoRacks Frames into the EXPRESS Rack Locker, cleaning of the air inlet filter (as necessary), and data retrieval (as needed) during the mission.