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Effect of Microgravity and Radiation on Eukaryotic, Endothelial and Osteoblasts (CIS)
03.22.12

Overview | Description | Applications | Operations | Results | Publications | Images

Experiment/Payload Overview

Information provided courtesy of the Energia website.
Brief Summary

CIS (Cells in Space) is a Malaysian Space Agency (Angkasa) experiment that evaluates the affects of the microgravity environment on cancer cells.

Principal Investigator

Information Pending

Co-Investigator(s)/Collaborator(s)

Information Pending

Payload Developer Information Pending

Sponsoring Space Agency

Roscosmos

Sponsoring Organization:

Information Pending

ISS Expedition Duration:

October 2007 - April 2008



Expeditions Assigned

16

Previous ISS Missions

Information Pending

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Experiment/Payload Description

Research Summary

Information Pending

Description

In CIS experiment three different research projects are to be performed, Eukaryotic Cells in Space (ECIS), Endothelial cells in microgravity environment (Huvec) and Osteoblastic cellular functions in micro-gravity environment (Osteo).

To determine the effects of spaceflight (specifically, microgravity and space radiation) on gene expression and morphology of eukaryotic cells (ECIS). To investigate the effects of micro-gravity on endothelial dysfunction and inflammation (Huvec). To study the effects of space radiation on DNA and determine spaceflight induced chromosomal rearrangement in osteoblasts due to exposure to spaceflight environment (Osteo).

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Applications

Space Applications

Information Pending

Earth Applications

Information Pending

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Operations

Operational Requirements

Cultivation of cell cultures onboard the ISS RS (ECIS ). Cultivation of endothelial cells onboard the ISS RS (Huvec). Cultivation of osteoblastic cells onboard the ISS RS (Osteo).

Operational Protocols

Three similar CIS Kits: CIS Kit 1, CIS Kit 2 and CIS Kit 3 with biological materials. Each of the first two CIS Kits consists of four FPA sets (twelve FPA biocontainers) and one HOBO data recorder. CIS Kit 3 consists of six FPA sets (twelve FPA biocontainers) and one HOBO data recorder. Each FPA set of CIS Kit 1 and CIS Kit 2 includes three PFAs, and CIS Kit 3 contains FPA sets consisting of two FPAs.

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Results/More Information

CIS KIT 1, CIS KIT 2 and CIS KIT 3 returned to the ground with grown biomaterials and accompanying photo information on a flash card.

To complete the objective of ECIS project eight FPA SETs (24 FPAs) will be used, in which Chamber A is filled with a complete growth medium, Chamber B is filled with cell suspension and Chamber C with a fixative or medium.

To complete the objective of Huvec project three FPA SETs (6 FPAs) will be used, in which Chamber A is filled with a culture medium, Chamber B is filled with an additional culture growth medium and Chamber C with a fixative or medium.

To complete the objective of Osteo project three FPA SETs (6 FPAs) will be used, in which Chamber A is filled with a culture mixture (osteoblast), Chamber B is filled with a medium (culture medium with serum) and Chamber C with a fixative or medium.

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Related Web Sites
  • Energia - Science Research on the ISS Russian Segment

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    Publications

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    Ground Based Results Publications

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    ISS Patent Publications

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    Related Publications

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    Images

    Information provided by the investigation team to the ISS Program Scientist's Office.
    If updates are needed to the summary please contact JSC-ISS-Program-Science-Group. For other general questions regarding space station research and technology, please feel free to call our help line at 281-244-6187 or e-mail at JSC-ISS-Payloads-Helpline.
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