Cell Culture Module - Effect of Microgravity on Wound Repair: In Vitro Model of New Blood Vessel Development (CCM-Wound Repair) is Department of Defense Space Test Program research that uses cell culture in micrgravity as a model of wound healing. This investigation is directed at the use of adipose derived adult stem cells for use in injury repair and how the microgravity alters new blood vessel development which is a key component of wound and tissue repair.Principal Investigator(s)
United States Department of Defense Space Test Program, Johnson Space Center, Houston, TX, United States
Walter Reed Army Institute of Research, Silver Spring, MD, United States
National Aeronautics and Space Administration (NASA)Sponsoring Organization
Department of Defense (DoD) - RetiredISS Expedition Duration
April 2007 - October 2007
15Previous ISS Missions
This experiment model has not previously flown in the CCM, although the CCM has flown on previous space shuttle missions.
Previous microgravity studies indicate that space flight diminishes the wound repair process. Cell Culture Module - Effect of Microgravity on Wound Repair: In Vitro Model of New Blood Vessel Development (CCM-Wound Repair) used primary endothelial cells derived from adipose tissue as a wound repair model to study and potentially mitigate the effects of microgravity on wound repair. When Endothelial cells, which are found lining all blood vessels, are in
culture they form cords and tubes that are precursors to new blood vessels. It is believed that the processes of new blood vessel formation and maturation will be impaired when the cells are cultured in microgravity, as compared to
Earth-based cultures. It is also expected to see differences in gene expression when the cultures are subjected to genomic analysis.
The endothelial cells were seeded onto custom flatbed bioreactors and perfused. Growth hormone (VEG-F) was injected into half of the samples preflight, and all samples were fixed during space flight. It is suspected that those exposed to growth hormone will show improved blood vessel formation and maturation compared to those without growth hormone.
Astronauts traveling to the moon or Mars in microgravity may experience injury or trauma, initiating the wound healing process. The blood vessel experiment will help determine a potential treatment to minimize any negative effects of microgravity on wound healing and blood vessel formation.Earth Applications
This unique cell culture in microgravity serves as a model system for understanding necrosis of tissue following severe injuries. Using adipose derived adult stem cells may accelerate new blood vessel development which is a key component of wound and tissue repair. Results will be applied to help prevent the loss of limbs following severe injuries with life-changing benefits in military and civilian applications.
Four rails with individual flowpaths and biochambers will be housed inside the CCM hardware. Two of the rails will house three individual biochambers/flowpaths each with primary endothelial cells. The rails will provide growth media, environment monitors and cell fixation capabilities.Operational Protocols
The CCM investigation is self contained and requires crew interaction for activation, daily status checks and re-entry. Rails will contain cell lines treated with different wound repair factors or growth hormones. Following return to Earth, the rails will be returned to the investigator for in-depth analysis.
Preliminary results from CCM-Wound Repair show that the cells were successfully cultured and returned to Earth. Data analysis is ongoing to determine deviations in cell pathology between flight and ground cells used to characterize the microgravity induced stresses on the flight samples. (Evans et al. 2009)
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Vandenburgh HH, Chromiak JA, Shansky J, LeMaire J, Del Tatto M. Space travel directly induces skeletal muscle atrophy. Federation of American Societies for Experimental Biology Journal. 1999; 13: 1031-1038.