The Avian Development Facility (ADF) is a middeck-locker-equivalent facility containing two independent specimen centrifuges that can be operated to produce inertial accelerations equivalent to 0 to 5 G under feedback control. Each centrifuge has 18 sample containers, each of which was designed to hold one Japanese-quail egg for a study of the effects of microgravity on the development of fixed or live embryos. A chemical robot injects solutions into the sample containers under triple-containment conditions.Facility Manager(s)
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National Aeronautics and Space Administration (NASA)Expeditions Assigned
4Previous ISS Missions
The first mission of the Avian Development Facility was STS-108/UF-1 in December 2001.
The Avian Development Facility (ADF) provides capacity for 36 egg holders on two identical carousels. Each carousel rotates independently, and each egg holder rotates independent of carousel rotation. The carousels are served by a chemical robot that injects reagent solutions into one sample at a time on a predetermined schedule or by remote control.
The ADF provides a snapshot of embryogenesis (embryo development) in space using avian egg specimens as a biological model. Avian eggs are ideally suited for microgravity research because they are self-contained, self-sustaining, and unaffected by maternal host. The ADF's single middeck locker size, which is compatible with space shuttle middeck, Spacehab, and International Space Station, supports flight experiments with Japanese quail or other small eggs, and can easily be modified to accommodate fish, plants, insects, or cells.
Any sample that can be contained within a spherical plastic container can be substituted for an avian egg in the versatile ADF holders, or the egg holders can be replaced with solid cylindrical containers. ADF provides advanced telerobotics and teleoperations to minimize dependence on crew time and significantly improve the science return of microgravity life science investigations.
The ADF is fully programmable in a closed environment system and can be programmed to monitor and control temperature, relative humidity, oxygen supply, and carbon dioxide removal. The ADF provides full telemetry capabilities to downlink data and uplink commands for real-time telescience and telerobotic experiment manipulation. The ADF maintains optimal incubation conditions for embryos from Day 0 until the eggs hatch. The ADF can delay onset of embryo development by programming the facility for a low-temperature environment.
ADF also provides concomitant controls at various levels of artificial gravity by programming one or both of the carousels to rotate. Independent of carousel rotation, the ADF turns each egg about its longitudinal axis automatically at programmable time intervals. The ADF automatically injects any specimen with a chemical fixative (or other liquid) upon active command or on a preprogrammed schedule.
Prior to launch, specimens or samples must be loaded into the hardware; a timeline is established for operations, such as temperature profiles, fluid injections, centrifuge speeds, automated gas exchanges, and entered into the hardware's main computer using its graphical user interface. A catalogued sample or specimen is loaded into each of 18 sample holders on one centrifuge, and their control counterparts are loaded into each of the 18 sample holders on the other centrifuge. The hardware is late loaded into the spacecraft; on orbit, the investigation is initiated as designated by the investigator. One centrifuge is rotated to produce an inertial acceleration of 1 G (Earth's gravity), while the other is not rotated or provides a partial-gravity environment. During the incubation phase, it is possible to chemically fix single living specimens.
Two principal investigators' experiments were supported on space shuttle flight STS-108. Dr. J. D. Dickman (Washington University, St. Louis) studied the development of vestibular apparatus components and found them to be significantly less innervated when developing in low gravity. Control samples were on the ADF 1-G centrifuge aboard the space shuttle. Dr. Stephen B. Doty (Hospital for Special Surgery, New York) designed and completed a study of musculoskeletal development and found few, if any, differences in the embryonic bone development of embryos flown in 0-G and embryos flown in 1-G .
Vellinger J, Ormsby R, Thomas N, Kennedy D, Hudson D, Todd P. Providing an Optimal Environment Utilizing the Avian Development Facility for Research in Microgravity. Society of Automotive Engineers Technical Paper Series, Warrendale, PA; 2001