Mechanisms of Gravity Resistance in Plants From Signal Transformation and Transduction to Response (Resist Tubule) - 08.20.14

Overview | Description | Applications | Operations | Results | Publications | Imagery
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Mechanisms of Gravity Resistance in Plants - From Signal Transformation and Transduction to Response (Resist Tubule) clarifies the mechanisms of gravity resistance. Gravity resistance is a principal gravity response in plants, and plays an important role in the transition of plant ancestors from an aquatic environment to a terrestrial environment (about 450 million years ago), and in the consequent establishment of land plants.  This present study  clarifies the mechanisms of gravity resistance, in particular the processes from signal transformation and transduction, to response.
 

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The following content was provided by Takayuki Hoson, Ph.D., and is maintained in a database by the ISS Program Science Office.
Information provided courtesy of the Japan Aerospace and Exploration Agency (JAXA).

Experiment Details

OpNom

Principal Investigator(s)

  • Takayuki Hoson, Ph.D., Osaka City University, Osaka, Japan

  • Co-Investigator(s)/Collaborator(s)
  • Takashi Hashimoto, Ph.D., Nara Institute of Science and Technology, Nara, Japan
  • Kazuyuki Wakabayashi, Ph.D., Osaka City University, Osaka, Japan
  • Koichi Soga, Ph.D., Osaka City University, Osaka, Japan
  • Hirofumi Hashimoto, JAXA, Kanagawa, Japan
  • Shunya Muranaka, Yokohama City University, Japan
  • Seiji Sonobe, Ph.D., University of Hyogo, Hyogo, Japan
  • Tsuyoshi Sakaki, Tokai University, Japan

  • Developer(s)
    JAXA Institute of Space and Aeronautical Science, Sagamihara, , Japan

    Sponsoring Space Agency
    Japan Aerospace Exploration Agency (JAXA)

    Sponsoring Organization
    Information Pending

    Research Benefits
    Information Pending

    ISS Expedition Duration
    May 2012 - September 2014

    Expeditions Assigned
    31/32,33/34,35/36,37/38,39/40

    Previous ISS Missions
    Information Pending

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    Experiment Description

    Research Overview

    • Mechanisms of Gravity Resistance in Plants - From Signal Transformation and Transduction to Response (Resist Tubule) examines modifications of nature of cellular components responsible for gravity resistance under microgravity conditions in space.


    • The contribution of membrane rafts and cortical microtubules to gravity resistance is confirmed. The contribution of cortical microtubules is quantified.


    • The basic mechanism of gravity resistance will be clarified, which greatly deepens our knowledge of plant responses to the gravity signal.

    Description

    Gravity resistance is a principal gravity response in plants, comparable to gravitropism, and has played an important role in the transition of plant ancestors from an aquatic environment to a terrestrial environment about 450 million years ago and in the consequent establishment of land plants. Nevertheless, only limited information has been obtained for the mechanism of gravity resistance. The ground-based experiments using hypergravity conditions have shown that membrane sterol rafts and cortical microtubules play an important role in signal transformation and transduction and that an increase in cell wall rigidity as the final response is mediated by the structural continuum or physiological continuity of these cellular components. The present study will clarify the mechanisms of gravity resistance, in particular the processes from signal transformation and transduction to response.

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    Applications

    Space Applications

    Information on the mechanism of gravity resistance, brought about by the present study, will enable efficient plant production indispensable for human life in space.
     

    Earth Applications

    Information on the mechanism of gravity resistance, brought about by the present study, will enable efficient plant production indispensable for human life not only in space, but also on earth.
     

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    Operations

    Operational Requirements

    Run1: Four kinds of mutants are grown in Chamber A and gotten fluorescence images using CB microscope and optional CCD camera. Run3: Three kinds of mutants and wild strains are grown in Plant Exp Unit. Plants growth images are acquired daily from 4 chambers for 32 days. After 32 days of growth, crew retrieves Plant Chambers. Eight KSC fixation tubes are stowed at 2 C for 5 days and transferred to -95C and recovered in -95C.

    Operational Protocols

    Run1: Four Chamber A containing dry Arabidopsis seeds are lunched with chamber A holders at cooled temperature. Two kinds of mutants are grown in one chamber A, total 4 kinds of mutants in 2 chambers. Crew will give water to the chambers and insert into MELFI at +2C for germination start. After 4 days , the chambers will be moved to CBEF for growth for 3 days. After growth, plant seedlings in the chambers are observed using fluorescence microscope in CB, combined with EMCCD camera.
    Run3: Four Plant culture chambers containing dry Arabidopsis seeds are launched in Plant Exp Unit (1 chamber in 1 Unit). Three kinds of mutants and wild strains are grown in Plant Exp Unit automatically with daily image acquiring. After 32 days of growth, crew retrieves Plant Chamber and harvests plants, then fixed using KSC fixation tubes. KSC fixation tubes are stowed at 2 C for 5 days and transferred to -95C and recovered in -95C. 

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    Results/More Information

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    Related Websites

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    Imagery

    image NASA Image: ISS033E011704 - This image shows the Growth Chambers containing Arabidopsis seeds.
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