Veggie hardware validation test (Veg-01) (Veg-01) - 03.25.14
ISS Science for Everyone
Science Objectives for Everyone
The Veg-01 hardware validation test investigation utilizes the Veggie facility on ISS. This investigation will assess on-orbit function and performance of the Veggie, and focus on the growth and development of ‘Outredgeous’ Lettuce (Lactuca sativa ) seedlings in the spaceflight environment and the effects of the spaceflight environment on composition of microbial flora on the Veggie-grown plants and the Veggie facility. Lettuce plants are harvested on-orbit, frozen at <-80oC and returned to the ground for post-flight evaluation. Microbial sampling swabs will be taken of the Veggie facility and plant material, frozen and returned to the ground for environmental microbiological examination. Rooting pillows and water sample syringes will also be returned for microbial sampling and root analysis.
Science Results for Everyone
Kennedy Space Center, , FL, United States
Sponsoring Space Agency
National Aeronautics and Space Administration (NASA)
Human Exploration and Operations Mission Directorate (HEOMD)
ISS Expedition Duration
March 2014 - September 2014
Previous ISS Missions
Assess ease of set-up and operation of Veggie hardware and science
Assess capacity for Veggie hardware and pillows to effectively germinate seeds
Assess capacity for Veggie hardware and pillows to effectively sustain plant growth and adequate media moisture
Compare growth in different media combinations
Collect environmental data via data logger (e.g. HOBO)
Record plant development with photographs
Assess crew handling aspects of Veggie (planting, daily plant checks and reservoir refilling, harvesting, pillow disposal, sanitation) and determine effectiveness of established crew procedures
Assess crew psychological benefits of plant growth and crew acceptance of Veggie operations (questionnaire)
Analyze microbial status and assess sanitation methods
The overall goal of Veg-01 is to demonstrate proof-of concept for the Veggie plant growth chamber and the planting pillows. This research builds upon hardware development via an SBIR grant to ORBITEC for the initial prototype Veggie units with subsequent hardware development for next-generation units. Both ORBITEC and KSC have been involved in plant growth optimization of the Veggie hardware and testing and collaboration have resulted in the development of the pillow planting concept to interface with the Veggie hardware. Through numerous tests the VEG-01 science team has refined the pillow concept and selected growth media and fertilizers, plant species, materials, and protocols for using the pillow concept in Veggie to grow healthy plants that can provide crew with food and recreation. The pillow concept is designed to be low mass, modular, require no additional energy and be very low maintenance. Pillows of different sizes have been designed to accommodate a wide variety of plant types and different types of growing media.
The primary goal of the Veg-01 testing will be to demonstrate plant growth in the Veggie hardware using lettuce as a test species. Plants will be grown in two different sizes of arcillite, a calcined clay media. This test will help us compare root zones of the two media sizes to determine water and root distribution in the different sized-particles to provide recommendations for future Veggie investigations. Shoot samples will also provide information on any growth anomalies when compared with ground controls and will provide information on microbial growth and food safety. Photographs will be used to assess plant growth rates and plant health. A data logger will record the environment within the Veggie hardware. Crew questionnaires will provide insight into the appropriateness and thoroughness of the crew procedures for Veggie hardware and plant growth operations.
Pillows are single use and thus reduce the chances of microbial contamination of the Veggie hardware and produce. A major aspect of the proof of concept flight, Veg-01, is to collect baseline microbial data from plants and pillows grown on ISS. Ground testing has demonstrated very low microbial levels on lettuce plants grown in Veggie-relevant conditions. Discussions with space microbiologists, flight surgeons, and space food technologists at JSC indicate that if microbial levels are sufficiently low the crew could consume the fresh produce without sanitizing. For crops that naturally have higher levels of microorganisms (e.g. radishes, which grow in contact with water and nutrients) a space-rated produce sanitation method must be developed and tested.
The baseline data collected from the Veg-01 flight will be a resource for future Veggie investigations. This information will provide data on necessary procedural changes, hardware upgrades or horticultural options as Veggie becomes an integral part of ISS expeditions in the future.
Crop testing has been an important part of NASA’s Advanced Life Support (ALS) and Controlled Ecological Life Support System (CELSS) Programs for over 30 years (Wheeler et al., 2001). These studies historically focused on optimizing productivity of crops in controlled environments, with an ultimate objective of implementing a bioregenerative life support system. The best initial opportunity for testing crop production in space is on the International Space Station. Although volume and power constraints limit the size of plant systems, the ISS can still provide a valuable flight setting to test many issues related to crop production. Key among these is (1) the value of adding fresh (perishable) foods on a regular basis to the crew’s diet, and (2) the potential for providing a positive effect on the crew’s well-being by having plants in their environment. To date, no large scale crop production tests have been conducted in space, hence the need for a functioning flight system with more growing volume than previous experimental systems.
As with all basic research, an improved understanding of the basic growth and environmental response phenomena of living organisms has important implications for improving growth and biomass production on Earth, thus benefitting the average citizen. Veggie technology might also be readily adaptable to hort-therapy and recreational activities for elderly or disabled individuals.
Set up and activation of Veggie facility
Priming of plant pillows
Initial filling of the Veggie reservoir and initial water sampling.
Wick opening after 3 days
Plant thinning after 7 days
Experiment run duration: 28 days with harvest, swab sampling, water sampling and pillow sampling
Time between harvest and MELFI insertion: < 1 hours
Weekly photos of plant growth requested
Harvest photos required; request live video of harvest operations when possible
Return Consists of
Microbial Swabs , Plant samples, water sample syringes and plant pillows return at -95°C in GLACIER
Data logger returned at +4 in Cold Bag
Photos returned via downlink
Sanitation of Veggie hardware following harvest
Veggie will be activated and the desired photoperiod, light levels, and fan speed programmed in via the control panel. The bellows baseplate assembly will be removed from the Veggie hardware. The root mat will then be placed in the Veggie bellows assembly. Six plant pillows will then be destowed and placed on the root mat in designated positions. The watering syringe assembly and water bag will be used to pull water from PWD and inject it into the plant pillow priming fitting to initially wet each pillow. The bellows and baseplate assembly will be reinstalled in the Veggie hardware and the bellows will be attached to the light cap. Water from PWD will be used for the initial filling of the root mat reservoir. After filling the root mat, and initial water sample will be taken and placed in MELFI at -80°C. Initial photos of the setup will be taken. The Veggie unit will be checked daily and additional water from PWD will be added as needed using the watering kit syringe. Wicks will be opened on day 3 after initiation. Plants will be thinned to one plant per pillow after 7 days. Weekly photos of plant growth will be taken and downloaded to the ground. After 28 days, final photographs and microbial samples will be taken, and plant samples will be collected (live video of harvest operations would be desirable). The lettuce plants will be harvested by cutting the stem at the interface of the plant pillow and wrapped in foil sheets, placed in a sample return bag, and placed in MELFI at < -80°C. Two plant pillows, a post-harvest water sample, and microbial swab samples should be placed in MELFI at < -80°C less than 1 hour after collection. Remaining pillows should be disposed of by placing in plastic trash bags. The root mat will be discarded. Prosan wipes followed by dry wipes will be used to clean and sanitize the interior of the Veggie unit and then discarded. Final photographs will be sent to the PIs via downlink. Science samples including the data logger will be returned on the first available flight after harvest, the data logger will be returned at +4°C in a Cold Bag and plant, water, swab and pillow samples return at -95°C in GLACIER.
Ground Based Results Publications