Preliminary Advanced Colloids Experiment - Light Microscopy Module: Biological Samples (PACE-LMM-Bio) - 07.01.15

Overview | Description | Applications | Operations | Results | Publications | Imagery

ISS Science for Everyone

Science Objectives for Everyone
Preliminary Advanced Colloids Experiment - Light Microscopy Module: Biological Samples (PACE-LMM-Bio) is a NASA Rapid Turn Around (RTA) engineering proof-of-concept proposal in preparation for the Advanced Colloids Experiment (ACE). In Bio, crewmembers image three-dimensional biological sample particles, tissue samples and live organisms. The goal of this experiment is to indicate the microscope’s capabilities for viewing biological specimens.
Science Results for Everyone
Information Pending

The following content was provided by Jacob N. Cohen, Ph.D., and is maintained in a database by the ISS Program Science Office.
Experiment Details

OpNom:

Principal Investigator(s)
Jacob N. Cohen, Ph.D., Ames Research Center, Moffett Field, CA, United States

Co-Investigator(s)/Collaborator(s)
Information Pending

Developer(s)
ZIN Technologies Incorporated, Cleveland, OH, United States

Sponsoring Space Agency
National Aeronautics and Space Administration (NASA)

Sponsoring Organization
Human Exploration and Operations Mission Directorate (HEOMD)

Research Benefits
Information Pending

ISS Expedition Duration
September 2010 - September 2011

Expeditions Assigned
25/26,27/28

Previous ISS Missions
Increments 25/26 is the first for this investigation.

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Experiment Description

Research Overview

  • Preliminary Advanced Colloids Experiment - Light Microscopy Module: Biological Samples (PACE-LMM-Bio) expands the utility of the Light Microscopy Microscope hardware and provides a resource for biological studies on the International Space Station (ISS).


  • Researchers are conducting this technology demonstration to determine the biologically useful range of operation of the LMM.


  • This experiment prepares the LMM for use in future biology experiments which require microscopic imaging.

Description
There are three tests proposed in Preliminary Advanced Colloids Experiment - Light Microscopy Module: Biological Samples (PACE-LMM-Bio). The first test uses micron size beads with different colors and one size that fluorescence. In the second test, the crew uses premade slides of tissue and small organisms that have already been stained and fixed to check for resolution and contrast capability on fixed biological samples. In the final test, the crew observes C. elegans, planaria and cell cultures at ambient temperature. They will observe these organisms and cells in real time. These worms are a commonly used model organism for cell development, behavior, genetics and radiation studies.

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Applications

Space Applications
PACE-LMM-Bio enables the use of LMM for biological studies while in space. This capability improves the ability to analyze the effects the space environment has on biological systems. Analysis of samples on the ISS removes the effects of return and may reduce the need for sample return. The PACE-LMM-Bio experiment demonstrates the ability of the Light Microscopy Microscope to perform fluid physics and biological experiments on ISS. PACE-LMM-Bio in conjunction with the first experiment performed in LMM (Constrained Vapor Bubble) demonstrates the magnification, lighting, data collection and remote commanding needed for ISS biological experiments.

Earth Applications
PACE-LMM-Bio enables the use of LMM for biological samples which may expand our understanding of the space environment effects on biological systems. Since all life evolved under the effects of gravity, the absence of gravity may identify novel pathways that are important for a fundamental understanding of life’s basic processes on Earth.

The biological research community needs a high-quality microscope for use in microgravity. This engineering experiment proposes to determine the capabilities of the LMM and establish what limitations exist regarding its use for biological studies.

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Operations

Operational Requirements
An ISS crewmember sets up the slides or OptiCell™ hardware in LMM Auxiliary Fluids Container. He or she uses premade slides of tissue and small organisms that have already been stained and fixed to check for resolution and contrast capability on fixed biological samples. The crewmember then focuses on observation of the living samples at ambient temperature over time. Ground-based commanding operates the LMM to acquire images of samples.

Operational Protocols
Five sessions are planned for a total of 12 hours of crew time. The first session requires the installation of air objectives & test slides. Then in the second session, operations are initiated with test slides, when the crew is awake and pursuing normal activities, asleep, and when they are exercising. Session 3 is the installation of OptiCell containing C. elegans: x 6 platens. Session 4 initiates OptiCell microscope operations, wake, sleep, exercise: x 6 platens. Session 5 cleans-up, stows, and shuts down microscope facility.

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Results/More Information

Information Pending

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Related Websites
ISS Research Project - Bio

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Imagery

image NASA Image: ISS029E011866 - NASA astronaut Mike Fossum (right), Expedition 29 commander, in front of the LMM (Light Microscopy Module) control box in preparation for another session with the PACE (Preliminary Advanced Colloids Experiment) hardware.
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image OPtiCell™ cell culture system.
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image Light Microscopy Module (LMM).
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