Preliminary Advanced Colloids Experiment: 100X Oil Test Target (PACE) - 12.03.13
Science Objectives for Everyone
Preliminary Advanced Colloids Experiment (PACE) is a technology demonstration, which sets the stage for the Advanced Colloid Experiment (ACE), by testing the Light Microscopy Module (LMM) in the International Space Station (ISS) environment. PACE establishes the capabilities of the LMM that are used for high resolution image magnification in ACE.
Science Results for Everyone
ZIN Technologies Incorporated, Cleveland, OH, United States
National Aeronautics and Space Administration (NASA)Sponsoring Organization
Human Exploration and Operations Mission Directorate (HEOMD)Research Benefits
Information PendingISS Expedition Duration
September 2010 - March 2011Expeditions Assigned
25/26Previous ISS Missions
ISS Expedition 23/24 is the first operation of PACE in microgravity.
- Preliminary Advanced Colloids Experiment (PACE) is a technology demonstration for the Advanced Colloids Experiment (ACE) planned for a future International Space Station (ISS) Expeditions.
- The vibration environment on the ISS is anticipated to have an impact on the magnifications possible with the Light Microscopy Module (LMM). The future operation of ACE will be determined by the extent to which these vibrations limit the resolution of the LMM.
- Both the air and the oil immersion objectives are tested at 50x, 63x, and 100x to establish the range of magnifications which can be used for ACE. The results of this experiment are used to determine the size of the particles to be used in ACE.
The data collected from Preliminary Advanced Colloids Experiment (PACE) ensures and optimizes the success of the Advanced Colloids Experiment (ACE). PACE determines which Light Microscopy Module (LMM) magnifications are usable before external vibrations blur the images. Both air and oil immersion microscope objectives (at 50x, 63x, and 100x magnifications) are used along with a microscope test target. The test target is used to resolve target images at different magnifications while operating with the vibrations that exist in an International Space Station (ISS) environment.
The test images are taken with the Active Rack Isolation System (ARIS) turned on (or active) and with it turned off. The results of these tests will determine the size of colloidal particles, which can be resolved by ACE, and therefore what size particles need to be synthesized for the experiments that have been proposed for ACE. Larger particles take much longer to form an equilibrium structure or state (doubling the particle diameter magnifies the experiment length by a factor of eight), so the use of the typical half-micron size spheres (which are the size of the wavelength of light) usually selected for use with an LMM type confocal microscope are ideal. The ACE scientists are required to create larger particles for use in ACE if the 100x oil immersion objectives do not provide sufficient resolution when external vibrations are present.
The ACE makes use of the PACE data, including the aging of gels and late collapse, phase separation competing with crystallization, asymmetric and seeded particle crystallization in the absence of gravitational jamming, 3-D growth and melting of crystals, and other proposed experiments.
The knowledge from PACE serves as preparation for the experimentation in ACE, which flies samples of colloid systems which serve as larger scale models of atomic systems. These samples are used to model the factors that influence phase-changes. Researchers anticipate these experiments will have a significant impact upon our understanding of fundamental physics as it pertains to how matter transitions from solid to liquid to gas.Earth Applications
PACE sets the groundwork for ACE, which later provides important data that cannot be obtained on Earth. This data can guide our understanding of phase separation (e.g., shelf-life, product collapse), and how it competes with crystallization to impact production (e.g., when making plastics). A better understanding of crystallization and phase-change processes could have an enormous commercial impact in a wide variety of industries.
An ISS crewmember sets up the hardware in LMM auxiliary fluids container. The crewmember injects immersion oil onto the test target and secures hardware for nominal operations. Ground teams then command the LMM to acquire images of the test target. Image acquisition is conducted during crew wake, sleep, and exercise periods with the FIR Active Rack Isolation System (ARIS) alternately active and disabled.Operational Protocols
Nine sessions are planned for a total of 14 hours of crew time.
- Session 1 - Install air objectives and test target.
- Session 2 - Initiate air test target operations during wake, sleep and exercise periods.
- Session 3 - Remove air objectives and test target, install oil objectives and test target, inject oil.
- Session 4 - Initiate oil test target operations during wake, sleep and exercise periods.
- Session 5 - Remove oil hardware, clean, install air objectives and test target, activate ARIS.
- Session 6 - Initiate air test target operations during wake, sleep and exercise periods.
- Session 7 - Remove air objectives and test target, install oil objectives and test target, inject oil.
- Session 8 - Initiate oil test target operations during wake, sleep and exercise periods.
- Session 9 - Clean-up and stow, shut down microscope facility.
Real-time image of 5 and 6 and their associated calibration bars on the PACE Target. This image was taken using the LMM 100x Lens immersed in the index matching oil. These numbers and bars are barely visible to the eye on the actual test target. Image courtesy of Glenn Research Center.
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Air Force Test Target used for the Light Microscopy Module (LMM) test target. Image provided courtesy of Glenn Research Center.
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PACE module with test target. Image provided courtesy of Glenn Research Center.
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100x image of the smallest subsection of the test target. This image was taken with an oil immersion objective, coupled directly to the target face. The image was taken with the camera set for Autoexpose, which yielded an exposure time of 1.61 ms. The image gives a contrast ratio of about 73%, which should yield a very effective method for measuring image variability due to motion or de-focus. Image provided courtesy of Glenn Research Center.
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