Gravity Related Genes in Arabidopsis - A (Genara-A) - 01.09.14
Science Objectives for Everyone Gravity Related Genes in Arabidopsis - A (Genara-A) seeks to provide an understanding of microgravity induced altered molecular activities which will help to find plant systems that compensate the negative impact on plant growth in space.
Science Results for Everyone Information Pending
EADS Astrium, Friedrichshafen, , Germany
Sponsoring Space Agency
European Space Agency (ESA)
ISS Expedition Duration:
March 2010 - March 2011
Previous ISS Missions
Genara-A is scheduled for its first operations on ISS Increment 23/24.
- Gravity Related Genes in Arabidopsis - A (Genara-A) will address the existence of gravity regulated genes, which affect the mechanism of gravisensing and the redistribution of plant growth hormones.
- For this purpose the growth of Arabidopsis shall be followed by optical observation of 1g reference samples and samples grown under microgravity.
- Genara-A will provide an understanding of microgravity induced altered molecular activities which will help to find plant systems that compensate the negative impact on plant growth in space, an aspect of special importance for the application of plant based systems in life support systems or as food source for long-duration space flights beyond low Earth orbit.
The existence of gravity regulated genes, whose expression depends (at least) upon the mechanism of gravisensing and the redistribution of hormones, shall be addressed properly in this experiment. In transgenic Arabidopsis plants, several biomonitors will report the distribution of IAA (plant hormone auxin [Indole-3-Acetic Acid]) and ABA (plant hormone [Abscisic Acid]) at the tissue level in microgravity or in the 1-g centrifuge.
Arabidopsis thalianaseedlings are launched dry state inside cultivation chambers (CCs). An impregnated filter paper below the seeds contains required nutrients for germination and growth, so that germination can be started by hydration with distilled water. A total of ninety-six CCs are flown.
For processing, the samples are provided with artificial air with a composition of oxygen, nitrogen, carbon dioxide and water according to the investigator specifications at a temperature of 22 degrees Celsius. The growth of the Arabidopsis is observed and recorded by the European Modular Cultivation System (EMCS) provided video observation and recording subsystems.
Following processing the CCs are removed from the ECs and either frozen at a temperature of -80 degrees Celsius in MELFI, and returned to ground in this condition in a suitable cold storage or filled with GUS-Assay and incubated in a incubator at a temperature of 37 degrees Celsius for 12 hours, first, and only afterwards frozen at -80 degrees C in MELFI. Currently GUS treatment is planned for 16 CCs.
Manzano AI, Matia I, Gonzalez-Camacho F, Carnero-Diaz E, van Loon JJ, van Loon JJ, Dijkstra CE, Dijkstra CE, Larkin O, Anthony P, Davey MR, Marco R, Medina F. Germination of Arabidopsis Seed in Space and in Simulated Microgravity: Alterations in Root Cell Growth and Proliferation. Microgravity Science and Technology. 2009; 21(4): 293-297.
Ground Based Results Publications
Valbuena MA, Manzano AI, van Loon JJ, van Loon JJ, Saez-Vasquez J, Carnero-Diaz E, Herranz R, Medina F. Auxin transport and ribosome biogenesis mutant/reporter lines to study plant cell growth and proliferation under altered gravity.The Joint Life Science Symposium, Aberdeen, United Kingdom; 2012 June 18-22 2 pp.
- The information on this page is provided courtesy of the ESA Erasmus Experiment Archive.
- The Arabidopsis Information Resource