NanoRacks Plate Reader (NanoRacks Plate Reader) - 12.03.13
Science Objectives for Everyone
NanoRacks Plate Reader is a laboratory instrument designed to detect biological, chemical or physical events of samples in microtiter plates. Microplate readers are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations.
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NanoRacks, LLC, Houston, TX, United States
National Aeronautics and Space Administration (NASA)Sponsoring Organization
National Laboratory (NL)ISS Expedition Duration
September 2011 - September 2014Expeditions Assigned
29/30,31/32,33/34,35/36,37/38,39/40Previous ISS Missions
- NanoRacks Plate Reader is a modified SpectraMax® M5e Multi-Mode Microplate Reader.
- The SpectraMax® M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes.
- NanoRacks Plate Reader possesses five modes, UV-Visible Absorbance, Fluorescence Intensity, Time-Resolved Fluorescence, Fluorescence Polarization, and Glow Luminescence, providing the benefit of multiple detection modes in one platform.
Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed optical pathlength cuvette. This temperature-independent normalization corrects for varying well volumes and can eliminate standard curves by allowing the calculation of concentrations directly from the absorbance with a known extinction coefficient, as exemplified with nucleic acid and protein quantitation.
For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the optical design provides the highest level of flexibility. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Assays can be better optimized by scanning across a range of wavelengths in increments as small as 1 nm. Up to 4 wavelength pairs can be read in one protocol for endpoint and kinetic measurements.
For luminescence, the NanoRacks Plate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. Operations
- A one-CTB microplate reader is installed near the Microgravity Science Glovebox. Crewtime is required for hardware installation, preparation of the test and science microplates, and stowage of the samples for return.
Information PendingResults Publications
Ground Based Results Publications
NASA Image: ISS034E005601 -
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NASA Image: ISS034E005602
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