NanoRacks Plate Reader (NanoRacks Plate Reader) - 12.10.14
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NanoRacks Plate Reader is a laboratory instrument designed to detect biological, chemical or physical events of samples in microtiter plates. Microplate readers are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations.
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OpNom: Plate Reader
NanoRacks LLC, Webster, TX, United States
Sponsoring Space Agency
National Aeronautics and Space Administration (NASA)
National Laboratory (NL)
ISS Expedition Duration
September 2011 - September 2014
Previous ISS Missions
NanoRacks Plate Reader is a modified SpectraMax® M5e Multi-Mode Microplate Reader. The SpectraMax® M5e Multi-Mode Microplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates. NanoRacks Plate Reader possesses five modes, UV-Visible Absorbance, Fluorescence Intensity, Time-Resolved Fluorescence, Fluorescence Polarization, and Glow Luminescence, providing the benefit of multiple detection modes in one platform.
Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed optical pathlength cuvette. This temperature-independent normalization corrects for varying well volumes and can eliminate standard curves by allowing the calculation of concentrations directly from the absorbance with a known extinction coefficient, as exemplified with nucleic acid and protein quantitation.
For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the optical design provides the highest level of flexibility. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Assays can be better optimized by scanning across a range of wavelengths in increments as small as 1 nm. Up to 4 wavelength pairs can be read in one protocol for endpoint and kinetic measurements.
For luminescence, the NanoRacks Plate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays.
- A one-CTB microplate reader is installed near the Microgravity Science Glovebox. Crewtime is required for hardware installation, preparation of the test and science microplates, and stowage of the samples for return.
Ground Based Results Publications
NASA Image: ISS034E005601 - View of the front panel of the NanoRacks Plate Reader prior to installation on the International Space Station.
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NASA Image: ISS034E005602 - The NanoRacks Plate Reader prior to installation on board the International Space Station.
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