NanoRacks Plate Reader (NanoRacks Plate Reader) - 07.15.14

Summary | Overview | Operations | Results | Publications | Imagery
ISS Science for Everyone

Science Objectives for Everyone

NanoRacks Plate Reader is a laboratory instrument designed to detect biological, chemical or physical events of samples in microtiter plates. Microplate readers are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations.

Science Results for Everyone
Information Pending



The following content was provided by Michael D. Johnson, and is maintained in a database by the ISS Program Science Office.

Facility Details

OpNom:

Facility Manager(s)

  • Michael D. Johnson, NanoRacks LLC, Houston, TX, United States

  • Facility Representative(s)
    Information Pending
    Developer(s)

    NanoRacks, LLC, Houston, TX, United States

    Sponsoring Space Agency
    National Aeronautics and Space Administration (NASA)

    Sponsoring Organization
    National Laboratory (NL)

    ISS Expedition Duration
    September 2011 - September 2014

    Expeditions Assigned
    29/30,31/32,33/34,35/36,37/38,39/40

    Previous ISS Missions
    Information Pending

    Availability

  • Onboard
  • ^ back to top



    Facility Description

    Facility Overview

    • NanoRacks Plate Reader is a modified SpectraMax® M5e Multi-Mode Microplate Reader.


    • The SpectraMax® M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes.


    • NanoRacks Plate Reader possesses five modes, UV-Visible Absorbance, Fluorescence Intensity, Time-Resolved Fluorescence, Fluorescence Polarization, and Glow Luminescence, providing the benefit of multiple detection modes in one platform.

    NanoRacks Plate Reader is a modified SpectraMax® M5e Multi-Mode Microplate Reader. The SpectraMax® M5e Multi-Mode Microplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates.

    Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed optical pathlength cuvette. This temperature-independent normalization corrects for varying well volumes and can eliminate standard curves by allowing the calculation of concentrations directly from the absorbance with a known extinction coefficient, as exemplified with nucleic acid and protein quantitation.

    For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the optical design provides the highest level of flexibility. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Assays can be better optimized by scanning across a range of wavelengths in increments as small as 1 nm. Up to 4 wavelength pairs can be read in one protocol for endpoint and kinetic measurements.

    For luminescence, the NanoRacks Plate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays.

    ^ back to top



    Operations

    Facility Operations

    • A one-CTB microplate reader is installed near the Microgravity Science Glovebox. Crewtime is required for hardware installation, preparation of the test and science microplates, and stowage of the samples for return.

    ^ back to top



    Results/More Information
    Information Pending

    Results Publications

    ^ back to top


    Ground Based Results Publications

    ^ back to top


    ISS Patents

    ^ back to top


    Related Publications

    ^ back to top


    Related Websites

    ^ back to top



    Imagery

    image

    NASA Image: ISS034E005601 -


    + View Larger Image


    image

    NASA Image: ISS034E005602


    + View Larger Image