Optimization of Heterological Expresssion in Yeasts-True Yeasts in Microgravity for Example Synthesis of the HBS Antigen of the Virus Hepatitis B (Antigen (Antigene)) - 07.15.14

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The following content was provided by L. A. Nesterova, and is maintained in a database by the ISS Program Science Office.

Experiment Details

OpNom

Principal Investigator(s)

  • L. A. Nesterova, Biopreparat, Russia

  • Co-Investigator(s)/Collaborator(s)
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    Developer(s)
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    Sponsoring Space Agency
    Russian Federal Space Agency (Roscosmos)

    Sponsoring Organization
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    Research Benefits
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    ISS Expedition Duration
    April 2006 - April 2009

    Expeditions Assigned
    13,14,15,16,17,18

    Previous ISS Missions
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    Experiment Description

    Research Overview
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    Description
    Compare specifics of heterologous expression of HbsAg gen of hepatitis B virus in yeasts S.cerevisiae under microgravity and gravity conditions and establish synthesis optimization techniques.

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    Applications

    Space Applications
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    Earth Applications
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    Operations

    Operational Requirements
    Container, Vial with an ampoule with lyophilized cultures, Tube with biosample vials, Temperature data logger.

    Operational Protocols
    Produce a clone collection of producer strain with heritable deviations in HbsAg gen expression level. Perform a genetic analysis and restriction mapping of plasmids and mutant clones.

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    Results/More Information
    Obtaining maximum complete potential characteristics of heterologous expression of HbsAg gen of hepatitis B virus in yeasts S.cerevisiae. Thereafter new principles of increasing producers? efficiency will be developed.

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    Related Websites
    Coordinating Scientific and Technical Council of the Russian Space Agency [Russian]
    Energia - Science Research on the ISS Russian Segment

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    Imagery