Optimization of Heterological Expresssion in Yeasts-True Yeasts in Microgravity for Example Synthesis of the HBS Antigen of the Virus Hepatitis B (Antigen (Antigene)) - 12.03.13
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Russian Federal Space Agency (Roscosmos)Sponsoring Organization
Information PendingResearch Benefits
Information PendingISS Expedition Duration:
April 2006 - April 2009Expeditions Assigned
13,14,15,16,17,18Previous ISS Missions
Compare specifics of heterologous expression of HbsAg gen of hepatitis B virus in yeasts S.cerevisiae under microgravity and gravity conditions and establish synthesis optimization techniques.
Information PendingEarth Applications
Container, Vial with an ampoule with lyophilized cultures, Tube with biosample vials, Temperature data logger.Operational Protocols
Produce a clone collection of producer strain with heritable deviations in HbsAg gen expression level. Perform a genetic analysis and restriction mapping of plasmids and mutant clones.
Obtaining maximum complete potential characteristics of heterologous expression of HbsAg gen of hepatitis B virus in yeasts S.cerevisiae. Thereafter new principles of increasing producers? efficiency will be developed.