Optimization of Heterological Expresssion in Yeasts-True Yeasts in Microgravity for Example Synthesis of the HBS Antigen of the Virus Hepatitis B (Antigen (Antigene)) - 06.18.15

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Science Objectives for Everyone
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Science Results for Everyone
Attack of the antigen clones! The surface antigen of the hepatitis B virus (HBsAg) indicates an active infection. This investigation sought to identify as many characteristics as possible of HBsAg in yeast and to compare those characteristics in microgravity and normal gravity. This could help scientists synthesize the antigen and produce clones with deviations in the antigen that could be passed on to offspring. The data confirmed the feasibility of breeding super-producers of the antigen, and showed that stability of HBsAg characteristics depends mainly on strain properties and experiment design and not on storage time on Earth or in space.

The following content was provided by L. A. Nesterova, and is maintained in a database by the ISS Program Science Office.
Experiment Details


Principal Investigator(s)
L. A. Nesterova, Biopreparat, Russia

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Sponsoring Space Agency
Russian Federal Space Agency (Roscosmos)

Sponsoring Organization
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Research Benefits
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ISS Expedition Duration
April 2006 - April 2009

Expeditions Assigned

Previous ISS Missions
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Experiment Description

Research Overview
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Compare specifics of heterologous expression of HbsAg gen of hepatitis B virus in yeasts S.cerevisiae under microgravity and gravity conditions and establish synthesis optimization techniques.

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Space Applications
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Earth Applications
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Operational Requirements
Container, Vial with an ampoule with lyophilized cultures, Tube with biosample vials, Temperature data logger.

Operational Protocols
Produce a clone collection of producer strain with heritable deviations in HbsAg gen expression level. Perform a genetic analysis and restriction mapping of plasmids and mutant clones.

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Results/More Information

Obtaining maximum complete potential characteristics of heterologous expression of HbsAg gen of hepatitis B virus in yeasts S.cerevisiae. Thereafter new principles of increasing producers efficiency will be developed.

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Related Websites
Energia - Science Research on the ISS Russian Segment

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