Optimization of Heterological Expresssion in Yeasts-True Yeasts in Microgravity for Example Synthesis of the HBS Antigen of the Virus Hepatitis B (Antigen (Antigene)) - 02.25.15

Overview | Description | Applications | Operations | Results | Publications | Imagery

ISS Science for Everyone

Science Objectives for Everyone
Information Pending
Science Results for Everyone
Attack of the antigen clones! The surface antigen of the hepatitis B virus (HBsAg) indicates an active infection. This investigation sought to identify as many characteristics as possible of HBsAg in yeast and to compare those characteristics in microgravity and normal gravity. This could help scientists synthesize the antigen and produce clones with deviations in the antigen that could be passed on to offspring. The data confirmed the feasibility of breeding super-producers of the antigen, and showed that stability of HBsAg characteristics depends mainly on strain properties and experiment design and not on storage time on Earth or in space.

The following content was provided by L. A. Nesterova, and is maintained in a database by the ISS Program Science Office.
Experiment Details

OpNom:

Principal Investigator(s)

  • L. A. Nesterova, Biopreparat, Russia

  • Co-Investigator(s)/Collaborator(s)
    Information Pending

    Developer(s)
    Information Pending

    Sponsoring Space Agency
    Russian Federal Space Agency (Roscosmos)

    Sponsoring Organization
    Information Pending

    Research Benefits
    Information Pending

    ISS Expedition Duration
    April 2006 - April 2009

    Expeditions Assigned
    13,14,15,16,17,18

    Previous ISS Missions
    Information Pending

    ^ back to top

    Experiment Description

    Research Overview
    Information Pending

    Description
    Compare specifics of heterologous expression of HbsAg gen of hepatitis B virus in yeasts S.cerevisiae under microgravity and gravity conditions and establish synthesis optimization techniques.

    ^ back to top

    Applications

    Space Applications
    Information Pending

    Earth Applications
    Information Pending

    ^ back to top

    Operations

    Operational Requirements
    Container, Vial with an ampoule with lyophilized cultures, Tube with biosample vials, Temperature data logger.

    Operational Protocols
    Produce a clone collection of producer strain with heritable deviations in HbsAg gen expression level. Perform a genetic analysis and restriction mapping of plasmids and mutant clones.

    ^ back to top

    Results/More Information

    Obtaining maximum complete potential characteristics of heterologous expression of HbsAg gen of hepatitis B virus in yeasts S.cerevisiae. Thereafter new principles of increasing producers efficiency will be developed.

    ^ back to top

    Related Websites
    Energia - Science Research on the ISS Russian Segment

    ^ back to top


    Imagery