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Crystalization of Biological Macromolecules and Generation of Biocrystal Film in the Conditions of Microgravity (Kristallizator)
04.26.13

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Overview | Description | Applications | Operations | Results | Publications | Imagery

Experiment Overview

This content was provided by I. S. Lubutin, Ph.D., and is maintained in a database by the ISS Program Science Office.

Brief Summary

Kristallizator (Crystallizer) allows the growth of large protein crystals in orbit and allows better determination of the 3-dimensional structure of the crystals with applications in applied biology, medicine, and pharmacology. It is operated in the CRYOGEM-03 cooler.

Principal Investigator(s)

  • I. S. Lubutin, Ph.D., Russia
  • Co-Investigator(s)/Collaborator(s)

    Information Pending

    Developer(s)
    Information Pending

    Sponsoring Space Agency

    Russian Federal Space Agency (Roscosmos)

    Sponsoring Organization

    Information Pending

    Research Benefits

    Information Pending

    ISS Expedition Duration:

    October 2005 - September 2011

    Expeditions Assigned

    12,13,14,15,16,18,21/22,25/26,27/28

    Previous ISS Missions

    Information Pending

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    Experiment Description

    Research Overview

    Information Pending

    Description

    Study of physical processes of protein crystallization to produce the protein monocrystals perfect in structure suitable for X-ray structure analysis. Study of biocrystal films of volumetric solution on substrates using the artificial epitaxy effect.

    Development of the new generation hardware and techniques to crystallize a large amount of proteins in the interests of basic and applied biology, medicine, pharmacology and microelectronics.

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    Applications

    Space Applications

    Information Pending

    Earth Applications

    Information Pending

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    Operations

    Operational Requirements

    Module-1 is a device to grow the protein monocrystals. Module-3 is a device to grow bidimensional oriented films using the artificial epitaxy effect.

    Operational Protocols

    Test techniques in microgravity conditions to produce monocrystals of biological macromolecules and orient film-forming crystallites. Test the hardware, modes and conditions to grow the protein monocrystals and biological films. Estimate efficiency and reliability of the used hardware. Test the experiment timeline to organize the small-batch growing of biocrystals and films in microgravity conditions.

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    Results/More Information

    Biocrystallization modules with the experiment results transported safely back to Earth for analysis, along with a log sheet with data of the experiment timeline. Also, the daily telemetry information of the air temperature in the accommodation area of biocrystallizers must be sent.

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    Results Publications

      Givargizov E, Grebenko AI, Zadorozhnaya LA, Melik-Adamyan VR.  Growth of biocrystalline films of PVC catalase in space using artificial epitaxy (graphoepitaxy). Journal of Crystal Growth. 2008; 310: 847-852. DOI: 10.1016/j.jcrysgro.2007.11.166.

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    Ground Based Results Publications

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    ISS Patents

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    Related Publications

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    Related Websites
  • Coordinating Scientific and Technical Council of the Russian Space Agency [Russian]
  • Energia - Science Research on the ISS Russian Segment
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    Imagery

    image Module 1.
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    image Module 3.
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    Information provided by the investigation team to the ISS Program Scientist's Office.
    If updates are needed to the summary please contact JSC-ISS-Program-Science-Group. For other general questions regarding space station research and technology, please feel free to call our help line at 281-244-6187 or e-mail at JSC-ISS-Research-Helpline.