Observation of the Chlorophyll Chromatography Process in Weightlessness (Khlorophyl) - 09.17.14

Overview | Description | Applications | Operations | Results | Publications | Imagery
ISS Science for Everyone

Science Objectives for Everyone
CCM will test the chromatography of chlorophyll (the separation of pigment in plants on chromatography paper by capillary action) from Brazilian native plants.

Science Results for Everyone
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Experiment Details

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Principal Investigator(s)
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Co-Investigator(s)/Collaborator(s)
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Developer(s)
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Sponsoring Space Agency
Russian Federal Space Agency (Roscosmos)

Sponsoring Organization
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Research Benefits
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ISS Expedition Duration
April 2006 - September 2006

Expeditions Assigned
13

Previous ISS Missions
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Experiment Description

Research Overview
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Description
Observation of the chlorophyll chromatography process in zero-g conditions with an educational view.

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Applications

Space Applications
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Earth Applications
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Operations

Operational Requirements
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Operational Protocols
Transmission of a mixture of pigments through a layer of colorless sorbent and registration of colored areas of chromagrams. Two labeled sealed experimental chambers are used in the experiment. Each chamber contains a sheet of chromatographic paper and a syringe filled with a chlorophyll solution. Chlorophyll is produced from leaves of a Brazilian plant and dissolved in ethyl alcohol. The chlorophyll chromatography activation is performed through solution injection to the chromatographic paper. As molecules in chlorophyll pigments have different characteristics (such as size and dissolubility), they move with different velocities over the paper sheet under the effect of solvent (ethylene glycol in this case). Chlorophyll has different pigments (carotenes, xanthophylls, chlorophyll A and chlorophyll B) of a typical color. When chlorophyll solution passes through chromatographic paper, molecules of each of pigments behave differently, that results in a phenomenon similar to a rainbow. As the gravity influences the capillary effect, different chromatograms will be received in zero-g conditions and on the ground. To register the chlorophyll chromatography process, photography performed by onboard aids of the Russian segment is used.

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Results/More Information
Information on the chlorophyll chromatography recorded on the flash card and registration sheets.

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Related Websites
Energia - Science Research on the ISS Russian Segment

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Imagery

image CCM kit which includes Experimental chambers (2 pc), CCM flash card and CCM registration sheets.
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