Effect of Microgravity and Radiation on Eukaryotic, Endothelial and Osteoblasts (CIS) - 05.13.15

Overview | Description | Applications | Operations | Results | Publications | Imagery

ISS Science for Everyone

Science Objectives for Everyone
CIS (Cells in Space) is a Malaysian Space Agency (Angkasa) experiment that evaluates the affects of the microgravity environment on cancer cells.
Science Results for Everyone
Information Pending

The following content was provided by S. Romanov, and is maintained in a database by the ISS Program Science Office.
Experiment Details

OpNom:

Principal Investigator(s)
S. Romanov, RSC Energia, Russia

Co-Investigator(s)/Collaborator(s)
Information Pending

Developer(s)
Information Pending

Sponsoring Space Agency
Russian Federal Space Agency (Roscosmos)

Sponsoring Organization
Information Pending

Research Benefits
Information Pending

ISS Expedition Duration
October 2007 - April 2008

Expeditions Assigned
16

Previous ISS Missions
Information Pending

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Experiment Description

Research Overview
Information Pending

Description
In CIS experiment three different research projects are to be performed, Eukaryotic Cells in Space (ECIS), Endothelial cells in microgravity environment (Huvec) and Osteoblastic cellular functions in micro-gravity environment (Osteo).

To determine the effects of spaceflight (specifically, microgravity and space radiation) on gene expression and morphology of eukaryotic cells (ECIS). To investigate the effects of micro-gravity on endothelial dysfunction and inflammation (Huvec). To study the effects of space radiation on DNA and determine spaceflight induced chromosomal rearrangement in osteoblasts due to exposure to spaceflight environment (Osteo).

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Applications

Space Applications
Information Pending

Earth Applications
Information Pending

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Operations

Operational Requirements
Cultivation of cell cultures onboard the ISS RS (ECIS ). Cultivation of endothelial cells onboard the ISS RS (Huvec). Cultivation of osteoblastic cells onboard the ISS RS (Osteo).

Operational Protocols
Three similar CIS Kits: CIS Kit 1, CIS Kit 2 and CIS Kit 3 with biological materials. Each of the first two CIS Kits consists of four FPA sets (twelve FPA biocontainers) and one HOBO data recorder. CIS Kit 3 consists of six FPA sets (twelve FPA biocontainers) and one HOBO data recorder. Each FPA set of CIS Kit 1 and CIS Kit 2 includes three PFAs, and CIS Kit 3 contains FPA sets consisting of two FPAs.

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Results/More Information

CIS KIT 1, CIS KIT 2 and CIS KIT 3 returned to the ground with grown biomaterials and accompanying photo information on a flash card.

To complete the objective of ECIS project eight FPA SETs (24 FPAs) will be used, in which Chamber A is filled with a complete growth medium, Chamber B is filled with cell suspension and Chamber C with a fixative or medium.

To complete the objective of Huvec project three FPA SETs (6 FPAs) will be used, in which Chamber A is filled with a culture medium, Chamber B is filled with an additional culture growth medium and Chamber C with a fixative or medium.

To complete the objective of Osteo project three FPA SETs (6 FPAs) will be used, in which Chamber A is filled with a culture mixture (osteoblast), Chamber B is filled with a medium (culture medium with serum) and Chamber C with a fixative or medium.

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Results Publications

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Ground Based Results Publications

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ISS Patents

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Related Publications

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Related Websites
Energia - Science Research on the ISS Russian Segment

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Imagery

image FPA biocontainer is a syringe-like device with a cylindrical glass container housed within a Lexan sheathe. The inside glass container has 3 compartments (chambers A, B, C) separated by two porous septa.
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