Effect of Microgravity and Radiation on Eukaryotic, Endothelial and Osteoblasts (CIS) - 08.20.14
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CIS (Cells in Space) is a Malaysian Space Agency (Angkasa) experiment that evaluates the affects of the microgravity environment on cancer cells.
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Sponsoring Space Agency
Russian Federal Space Agency (Roscosmos)
ISS Expedition Duration
October 2007 - April 2008
Previous ISS Missions
In CIS experiment three different research projects are to be performed, Eukaryotic Cells in Space (ECIS), Endothelial cells in microgravity environment (Huvec) and Osteoblastic cellular functions in micro-gravity environment (Osteo).
To determine the effects of spaceflight (specifically, microgravity and space radiation) on gene expression and morphology of eukaryotic cells (ECIS). To investigate the effects of micro-gravity on endothelial dysfunction and inflammation (Huvec). To study the effects of space radiation on DNA and determine spaceflight induced chromosomal rearrangement in osteoblasts due to exposure to spaceflight environment (Osteo).
Cultivation of cell cultures onboard the ISS RS (ECIS ). Cultivation of endothelial cells onboard the ISS RS (Huvec). Cultivation of osteoblastic cells onboard the ISS RS (Osteo).
Three similar CIS Kits: CIS Kit 1, CIS Kit 2 and CIS Kit 3 with biological materials. Each of the first two CIS Kits consists of four FPA sets (twelve FPA biocontainers) and one HOBO data recorder. CIS Kit 3 consists of six FPA sets (twelve FPA biocontainers) and one HOBO data recorder. Each FPA set of CIS Kit 1 and CIS Kit 2 includes three PFAs, and CIS Kit 3 contains FPA sets consisting of two FPAs.
CIS KIT 1, CIS KIT 2 and CIS KIT 3 returned to the ground with grown biomaterials and accompanying photo information on a flash card.
To complete the objective of ECIS project eight FPA SETs (24 FPAs) will be used, in which Chamber A is filled with a complete growth medium, Chamber B is filled with cell suspension and Chamber C with a fixative or medium.
To complete the objective of Huvec project three FPA SETs (6 FPAs) will be used, in which Chamber A is filled with a culture medium, Chamber B is filled with an additional culture growth medium and Chamber C with a fixative or medium.
To complete the objective of Osteo project three FPA SETs (6 FPAs) will be used, in which Chamber A is filled with a culture mixture (osteoblast), Chamber B is filled with a medium (culture medium with serum) and Chamber C with a fixative or medium.
Muid S, Froemming GR, Ali AM, Nawawi HM. Interleukin-6 and intercellular cell adhesion molecule-1 expression remains elevated in revived live endothelial cells following spaceflight. Malaysian Journal of Pathology. 2013 December; 35(2): 165-176. PMID: 24362480.
Muid S, Froemming GR, Manaf A, Muszaphar S, Yusoff KN, Nawawi HM. Changes in Protein and gene Expression of Adhesion Molecules and Cytokines of Endothelial Cells Immediately Following Short-term Spaceflight Travel. Gravitational and Space Biology. 2010 August; 23(2): S-1 - S-11.
Kapitonova MY, Muid S, Froemming GR, Yusoff KN, Othman S, Ali AM, Nawawi HM. Real space flight travel is associated with ultrastructural changes, cytoskeletal disruption and premature senescence of HUVEC. Malaysian Journal of Pathology. 2012; 34(2): 103-113. PMID: 23424772.
Ground Based Results Publications
Energia - Science Research on the ISS Russian Segment
FPA biocontainer is a syringe-like device with a cylindrical glass container housed within a Lexan sheathe. The inside glass container has 3 compartments (chambers A, B, C) separated by two porous septa.
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