Effect of Microgravity and Radiation on Eukaryotic, Endothelial and Osteoblasts (CIS) - 09.17.14

Overview | Description | Applications | Operations | Results | Publications | Imagery
ISS Science for Everyone

Science Objectives for Everyone
CIS (Cells in Space) is a Malaysian Space Agency (Angkasa) experiment that evaluates the affects of the microgravity environment on cancer cells.

Science Results for Everyone
Information Pending



The following content was provided by S. Romanov, and is maintained in a database by the ISS Program Science Office.

Experiment Details

OpNom

Principal Investigator(s)

  • S. Romanov, RSC Energia, Russia

  • Co-Investigator(s)/Collaborator(s)
    Information Pending
    Developer(s)
    Information Pending
    Sponsoring Space Agency
    Russian Federal Space Agency (Roscosmos)

    Sponsoring Organization
    Information Pending

    Research Benefits
    Information Pending

    ISS Expedition Duration
    October 2007 - April 2008

    Expeditions Assigned
    16

    Previous ISS Missions
    Information Pending

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    Experiment Description

    Research Overview
    Information Pending

    Description
    In CIS experiment three different research projects are to be performed, Eukaryotic Cells in Space (ECIS), Endothelial cells in microgravity environment (Huvec) and Osteoblastic cellular functions in micro-gravity environment (Osteo).

    To determine the effects of spaceflight (specifically, microgravity and space radiation) on gene expression and morphology of eukaryotic cells (ECIS). To investigate the effects of micro-gravity on endothelial dysfunction and inflammation (Huvec). To study the effects of space radiation on DNA and determine spaceflight induced chromosomal rearrangement in osteoblasts due to exposure to spaceflight environment (Osteo).

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    Applications

    Space Applications
    Information Pending

    Earth Applications
    Information Pending

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    Operations

    Operational Requirements
    Cultivation of cell cultures onboard the ISS RS (ECIS ). Cultivation of endothelial cells onboard the ISS RS (Huvec). Cultivation of osteoblastic cells onboard the ISS RS (Osteo).

    Operational Protocols
    Three similar CIS Kits: CIS Kit 1, CIS Kit 2 and CIS Kit 3 with biological materials. Each of the first two CIS Kits consists of four FPA sets (twelve FPA biocontainers) and one HOBO data recorder. CIS Kit 3 consists of six FPA sets (twelve FPA biocontainers) and one HOBO data recorder. Each FPA set of CIS Kit 1 and CIS Kit 2 includes three PFAs, and CIS Kit 3 contains FPA sets consisting of two FPAs.

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    Results/More Information
    CIS KIT 1, CIS KIT 2 and CIS KIT 3 returned to the ground with grown biomaterials and accompanying photo information on a flash card.

    To complete the objective of ECIS project eight FPA SETs (24 FPAs) will be used, in which Chamber A is filled with a complete growth medium, Chamber B is filled with cell suspension and Chamber C with a fixative or medium.

    To complete the objective of Huvec project three FPA SETs (6 FPAs) will be used, in which Chamber A is filled with a culture medium, Chamber B is filled with an additional culture growth medium and Chamber C with a fixative or medium.

    To complete the objective of Osteo project three FPA SETs (6 FPAs) will be used, in which Chamber A is filled with a culture mixture (osteoblast), Chamber B is filled with a medium (culture medium with serum) and Chamber C with a fixative or medium.

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    Results Publications

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    Ground Based Results Publications

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    ISS Patents

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    Related Publications

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    Related Websites
    Energia - Science Research on the ISS Russian Segment

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    Imagery

    image FPA biocontainer is a syringe-like device with a cylindrical glass container housed within a Lexan sheathe. The inside glass container has 3 compartments (chambers A, B, C) separated by two porous septa.
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