NASA - National Aeronautics and Space Administration

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Overview | Description | Applications | Operations | Results | Publications | Imagery

Experiment Overview


Brief Summary

CIS (Cells in Space) is a Malaysian Space Agency (Angkasa) experiment that evaluates the affects of the microgravity environment on cancer cells.

Principal Investigator(s)

Information Pending

Co-Investigator(s)/Collaborator(s)

Information Pending

Developer(s)
Information Pending

Sponsoring Space Agency

Russian Federal Space Agency (Roscosmos)

Sponsoring Organization

Information Pending

Research Benefits

Information Pending

ISS Expedition Duration

October 2007 - April 2008

Expeditions Assigned

16

Previous ISS Missions

Information Pending

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Experiment Description

Research Overview

Information Pending

Description

In CIS experiment three different research projects are to be performed, Eukaryotic Cells in Space (ECIS), Endothelial cells in microgravity environment (Huvec) and Osteoblastic cellular functions in micro-gravity environment (Osteo).

To determine the effects of spaceflight (specifically, microgravity and space radiation) on gene expression and morphology of eukaryotic cells (ECIS). To investigate the effects of micro-gravity on endothelial dysfunction and inflammation (Huvec). To study the effects of space radiation on DNA and determine spaceflight induced chromosomal rearrangement in osteoblasts due to exposure to spaceflight environment (Osteo).

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Applications

Space Applications

Information Pending

Earth Applications

Information Pending

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Operations

Operational Requirements

Cultivation of cell cultures onboard the ISS RS (ECIS ). Cultivation of endothelial cells onboard the ISS RS (Huvec). Cultivation of osteoblastic cells onboard the ISS RS (Osteo).

Operational Protocols

Three similar CIS Kits: CIS Kit 1, CIS Kit 2 and CIS Kit 3 with biological materials. Each of the first two CIS Kits consists of four FPA sets (twelve FPA biocontainers) and one HOBO data recorder. CIS Kit 3 consists of six FPA sets (twelve FPA biocontainers) and one HOBO data recorder. Each FPA set of CIS Kit 1 and CIS Kit 2 includes three PFAs, and CIS Kit 3 contains FPA sets consisting of two FPAs.

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Results/More Information

CIS KIT 1, CIS KIT 2 and CIS KIT 3 returned to the ground with grown biomaterials and accompanying photo information on a flash card.

To complete the objective of ECIS project eight FPA SETs (24 FPAs) will be used, in which Chamber A is filled with a complete growth medium, Chamber B is filled with cell suspension and Chamber C with a fixative or medium.

To complete the objective of Huvec project three FPA SETs (6 FPAs) will be used, in which Chamber A is filled with a culture medium, Chamber B is filled with an additional culture growth medium and Chamber C with a fixative or medium.

To complete the objective of Osteo project three FPA SETs (6 FPAs) will be used, in which Chamber A is filled with a culture mixture (osteoblast), Chamber B is filled with a medium (culture medium with serum) and Chamber C with a fixative or medium.

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Results Publications

Muid S, Froemming GR, Manaf A, Muszaphar S, Yusoff KN, Nawawi HM.  Changes in Protein and gene Expression of Adhesion Molecules and Cytokines of Endothelial Cells Immediately Following Short-term Spaceflight Travel. Gravitational and Space Biology. 2010 August; 23(2): S-1 - S-11.


Kapitonova MY, Muid S, Froemming GR, Yusoff KN, Othman S, Ali AM, Nawawi HM.  Real space flight travel is associated with ultrastructural changes, cytoskeletal disruption and premature senescence of HUVEC. Malaysian Journal of Pathology. 2012; 34(2): 103-113. PMID: 23424772.

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Ground Based Results Publications

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ISS Patents

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Related Publications

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Related Websites

Energia - Science Research on the ISS Russian Segment

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Imagery

FPA biocontainer is a syringe-like device with a cylindrical glass container housed within a Lexan sheathe. The inside glass container has 3 compartments (chambers A, B, C) separated by two porous septa.
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