Effect of Microgravity on Kinetic Properties of Enzymtic Reactions - Hydrolysis of Sucrose in Cerevisiae Cells of Saccharomycetes (SMEK) - 04.10.14
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Science Objectives for Everyone MEK (Effects of Micro-g on Fermentative Kinetics) is a study of the kinetics (rates) of enzymatic reactions with lipase and invertase (these break down fats and sugars respectively).
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Sponsoring Space Agency
Russian Federal Space Agency (Roscosmos)
ISS Expedition Duration
April 2006 - September 2006
Previous ISS Missions
To study the effect of microgravity on kinetic parameters of enzymatic reactions. To study differences in the hydrolysis of sucrose in cerevisiae cells of saccharomycetes.
Measurement of the enzymatic reaction activity by the example of three enzymes (invertase, lipase and immobilized lipase).
In design experimental equipment is implemented as a unit consisting of two parts: Unit of MEK chambers and MEK electronic board connected by a cable. The unit of MEK chambers houses three blocks isolated from each other and filled with experimental enzymes. Each experimental block accommodates 5 chambers with enzymes. The first experimental block houses 5 chambers with lipase.
The second block accommodates 5 chambers with immobilized (in silicon) lipase. The third block contains 5 chambers with invertase (of baker's yeast). Each ampoule is filled with olive oil of different concentration in water emulsion of polyvinyl alcohol in the range from 100 to 500 millimoles. The MEK electronic board is a control unit as a plug-in system board on which all information on the experiment progress is recorded.
Energia - Science Research on the ISS Russian Segment
The MEK experimental equipment includes, unit of MEK chambers (with a set of cables).
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