Effect of Microgravity on Kinetic Properties of Enzymtic Reactions - Hydrolysis of Sucrose in Cerevisiae Cells of Saccharomycetes (SMEK) - 12.03.13
Science Objectives for Everyone
MEK (Effects of Micro-g on Fermentative Kinetics) is a study of the kinetics (rates) of enzymatic reactions with lipase and invertase (these break down fats and sugars respectively).
Science Results for Everyone
Russian Federal Space Agency (Roscosmos)Sponsoring Organization
Information PendingResearch Benefits
Information PendingISS Expedition Duration
April 2006 - September 2006Expeditions Assigned
13Previous ISS Missions
To study the effect of microgravity on kinetic parameters of enzymatic reactions. To study differences in the hydrolysis of sucrose in cerevisiae cells of saccharomycetes.
Information PendingEarth Applications
Measurement of the enzymatic reaction activity by the example of three enzymes (invertase, lipase and immobilized lipase).Operational Protocols
In design experimental equipment is implemented as a unit consisting of two parts: Unit of MEK chambers and MEK electronic board connected by a cable. The unit of MEK chambers houses three blocks isolated from each other and filled with experimental enzymes. Each experimental block accommodates 5 chambers with enzymes. The first experimental block houses 5 chambers with lipase.
The second block accommodates 5 chambers with immobilized (in silicon) lipase. The third block contains 5 chambers with invertase (of baker's yeast). Each ampoule is filled with olive oil of different concentration in water emulsion of polyvinyl alcohol in the range from 100 to 500 millimoles. The MEK electronic board is a control unit as a plug-in system board on which all information on the experiment progress is recorded.
Exposed samples of three enzymes (invertase, lipase and immobilized lipase) in microgravity conditions and information recorded on the system board.
The MEK experimental equipment includes, unit of MEK chambers (with a set of cables).
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