Effect of Microgravity on Kinetic Properties of Enzymtic Reactions - Hydrolysis of Sucrose in Cerevisiae Cells of Saccharomycetes (SMEK) - 05.13.15

Overview | Description | Applications | Operations | Results | Publications | Imagery

ISS Science for Everyone

Science Objectives for Everyone
MEK (Effects of Micro-g on Fermentative Kinetics) is a study of the kinetics (rates) of enzymatic reactions with lipase and invertase (these break down fats and sugars respectively).
Science Results for Everyone
Information Pending

The following content was provided by S. Romanov, and is maintained in a database by the ISS Program Science Office.
Experiment Details

OpNom:

Principal Investigator(s)
S. Romanov, RSC Energia, Russia

Co-Investigator(s)/Collaborator(s)
Information Pending

Developer(s)
Information Pending

Sponsoring Space Agency
Russian Federal Space Agency (Roscosmos)

Sponsoring Organization
Information Pending

Research Benefits
Information Pending

ISS Expedition Duration
April 2006 - September 2006

Expeditions Assigned
13

Previous ISS Missions
Information Pending

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Experiment Description

Research Overview
Information Pending

Description
To study the effect of microgravity on kinetic parameters of enzymatic reactions. To study differences in the hydrolysis of sucrose in cerevisiae cells of saccharomycetes.

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Applications

Space Applications
Information Pending

Earth Applications
Information Pending

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Operations

Operational Requirements
Measurement of the enzymatic reaction activity by the example of three enzymes (invertase, lipase and immobilized lipase).

Operational Protocols
In design experimental equipment is implemented as a unit consisting of two parts: Unit of MEK chambers and MEK electronic board connected by a cable. The unit of MEK chambers houses three blocks isolated from each other and filled with experimental enzymes. Each experimental block accommodates 5 chambers with enzymes. The first experimental block houses 5 chambers with lipase.

The second block accommodates 5 chambers with immobilized (in silicon) lipase. The third block contains 5 chambers with invertase (of baker's yeast). Each ampoule is filled with olive oil of different concentration in water emulsion of polyvinyl alcohol in the range from 100 to 500 millimoles. The MEK electronic board is a control unit as a plug-in system board on which all information on the experiment progress is recorded.

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Results/More Information

Exposed samples of three enzymes (invertase, lipase and immobilized lipase) in microgravity conditions and information recorded on the system board.

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Related Websites
Energia - Science Research on the ISS Russian Segment

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Imagery

image The MEK experimental equipment includes, unit of MEK chambers (with a set of cables).
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