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Effect of Microgravity on Kinetic Properties of Enzymtic Reactions - Hydrolysis of Sucrose in Cerevisiae Cells of Saccharomycetes (SMEK)
04.26.13

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Overview | Description | Applications | Operations | Results | Publications | Imagery

Experiment Overview

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Brief Summary

MEK (Effects of Micro-g on Fermentative Kinetics) is a study of the kinetics (rates) of enzymatic reactions with lipase and invertase (these break down fats and sugars respectively).

Principal Investigator(s)

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Co-Investigator(s)/Collaborator(s)

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Developer(s)
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Sponsoring Space Agency

Russian Federal Space Agency (Roscosmos)

Sponsoring Organization

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Research Benefits

Information Pending

ISS Expedition Duration

April 2006 - September 2006

Expeditions Assigned

13

Previous ISS Missions

Information Pending

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Experiment Description

Research Overview

Information Pending

Description

To study the effect of microgravity on kinetic parameters of enzymatic reactions. To study differences in the hydrolysis of sucrose in cerevisiae cells of saccharomycetes.

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Applications

Space Applications

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Earth Applications

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Operations

Operational Requirements

Measurement of the enzymatic reaction activity by the example of three enzymes (invertase, lipase and immobilized lipase).

Operational Protocols

In design experimental equipment is implemented as a unit consisting of two parts: Unit of MEK chambers and MEK electronic board connected by a cable. The unit of MEK chambers houses three blocks isolated from each other and filled with experimental enzymes. Each experimental block accommodates 5 chambers with enzymes. The first experimental block houses 5 chambers with lipase.

The second block accommodates 5 chambers with immobilized (in silicon) lipase. The third block contains 5 chambers with invertase (of baker's yeast). Each ampoule is filled with olive oil of different concentration in water emulsion of polyvinyl alcohol in the range from 100 to 500 millimoles. The MEK electronic board is a control unit as a plug-in system board on which all information on the experiment progress is recorded.

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Results/More Information

Exposed samples of three enzymes (invertase, lipase and immobilized lipase) in microgravity conditions and information recorded on the system board.

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Related Websites
  • Energia - Science Research on the ISS Russian Segment

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    Imagery

    image The MEK experimental equipment includes, unit of MEK chambers (with a set of cables).
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    Information provided by the investigation team to the ISS Program Scientist's Office.
    If updates are needed to the summary please contact JSC-ISS-Program-Science-Group. For other general questions regarding space station research and technology, please feel free to call our help line at 281-244-6187 or e-mail at JSC-ISS-Research-Helpline.
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