Gene, Immune and Cellular Responses to Single and Combined Space Flight Conditions - A (TripleLux-A) studies the effects of space flight and radiation on the immune function of vertebrate cells in microgravity.Principal Investigator(s)
European Space Agency (ESA)Sponsoring Organization
Information PendingResearch Benefits
Information PendingISS Expedition Duration:
Information PendingPrevious ISS Missions
The aim of the Gene, Immune and Cellular Responses to Single and Combined Space Flight Conditions - A (TripleLux-A) experiment is to understand the mechanisms at the cellular level that impair vertebrate immune functions and enhance responses to radiation under space flight conditions. Specifically, TripleLux-A will study the ability of rodent macrophages (large white blood cells that ingests foreign material) from NR8383, ATCC# CRL-2192 to engulf zymosan (an insoluble carbohydrate that serves as an analogue of bacteria).
Phagocytosis (ingestion of foreign material) is the first line of defense against microbial infection by the immune system. Phagocytosis will be quantified using luminol as a detector for reactive oxygen species produced during the ingestion of zymosan by macrophages during the TripleLux-A investigation.
Conducting studies of the immune system during space flight will provide knowledge and understanding of the effects of space habitation on the immune system. The data from these studies will be used in assessing the cellular mechanisms underlying the aggravation of radiation responses and impairment of immune functions during space flight. Understanding such risks is essential in maintaining the health and performance of crewmembers during long-duration missions.Earth Applications
With greater understanding of the immune system in space, we can determine new countermeasures for people suffering from weakened immune systems.
Triplelux-A will require utilization of the MELFI and BioLab facilities onboard the ISS. To complete TripleLux-A operations one session of approximately 12 hours must be completed. Video containing the data of the TripleLux-A activites on orbit will be downloaded to Earth following investigation completion.Operational Protocols
Prior to TripleLux-A activation the following steps must be completed by ISS crewmembers, removal of the specimen from MELFI for thawing, injection of stock culture into the culture medium, reconstitution of the stock culture in fresh medium and measurement of the specimen viability. Crewmembers will then place the specimens in two (2) BioLab Advanced Experiment Containers (AECs) for processing of approximately 12-hours. Following completion of the experiment video data collect by BioLab will be returned to Earth for analysis.
Rabbow E, Rettberg P, Baumstark-Khan C, Horneck G. The SOS-LUX-LAC-FLUORO-Toxicity-test on the International Space Station (ISS). Advances in Space Research. 2003; 31(6): 1513-1524. DOI: 10.1016/S0273-1177(03)00086-3.