Multiple Orbital Bioreactor with Instrumentation and Automated Sampling (MOBIAS) - 07.15.14
ISS Science for Everyone
Science Objectives for Everyone Multiple Orbital Bioreactor with Instrumentation and Automated Sampling (MOBIAS) was designed to enable long-term cell culture growth on board the International Space Station.
Science Results for Everyone
University of Colorado at Boulder, BioServe Space Technologies, Boulder, CO, United States
Sponsoring Space Agency
National Aeronautics and Space Administration (NASA)
Human Exploration and Operations Mission Directorate (HEOMD)
ISS Expedition Duration
March 2001 - June 2002
Previous ISS Missions
MOBIAS was operated on ISS Expeditions 2 and 4.
- Multiple Orbital Bioreactor with Instrumentation and Automated Sampling (MOBIAS) is a fermentation, cell culture, and tissue engineering apparatus; which can be placed inside the Commercial Generic Bioprocessing Apparatus (CGBA).
- MOBIAS provides automated processing for biological experiments in microgravity through semi-continuous fed batch processing.
MOBIAS allows feed-batch processing with sampling and waste removal and passive gas exchange. MOBIAS provides a 50-ml total culture volume per tray. MOBIAS allows for fixation of samples (chemical and/or thermal) and a 350-ml media volume along with a 350-ml waste volume in a sterile environment using Teflon or silicone culture/sample bags. MOBIAS provides independent, adjustable temperature control for cultures and samples between 10 degrees C and 37 degrees C and supports six trays independently.
- Description MOBIAS is installed inside a CGBA to allow for automation and minimal crew time.
- Command and telemetry capabilities allowed real-time ground-based data monitoring and remote operations.
- Crew operations involved activation/deactivation, insertion/removal of hardware, status checks, and air filter maintenance.
Ground Based Results Publications
Post flight images show MOBIAS tray with waste bag and samples visible (dark substance indicates actinomycin D), close up of remaining viable culture from opened tray, and sample bags. Image courtesy of BioServe Space Technologies, University of Colorado - Boulder, Boulder, CO.
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Helping to develop and operate the MOBIAS payload provided me with an excellent opportunity to combine my background in microbiology with the engineering skills I learned while at BioServe. A substantial portion of my Ph.D. dissertation was devoted to this project, and this multidisciplinary experience helped me obtain my current position as a Post-Doctoral Scholar at Stanford University. Mike Benoit, Ph.D.
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