Gene, Immune and Cellular Responses to Single and Combined Space Flight Conditions - B (TripleLux-B) - 02.05.14

Overview | Description | Applications | Operations | Results | Publications | Imagery
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The Gene, Immune and Cellular Responses to Single and Combined Space Flight Conditions - B (TripleLux-B) studies the effects of space flight and radiation on the immune function of vertebrate cells in microgravity.
 

Science Results for Everyone
Information Pending



This content was provided by Peter-Diedrich Hansen, Ph.D., and is maintained in a database by the ISS Program Science Office.
Information provided courtesy of the Erasmus Experiment Archive.

Experiment Details

OpNom

Principal Investigator(s)

  • Peter-Diedrich Hansen, Ph.D., Berlin Institute for Technology, Berlin, Germany

  • Co-Investigator(s)/Collaborator(s)
  • Petra Rettberg, German Aerospace Center, Köln, Germany
  • Elke Rabbow, German Aerospace Center, Köln, Germany
  • Günter Reitz, Ph.D., German Aerospace Center, Köln, Germany
  • Bertold Hock, Ph.D., Technical University of Munich at Weihenstephan, Freising, Germany
  • Christa Baumstark-Khan, German Aerospace Center, Köln, Germany

  • Developer(s)
    Information Pending
    Sponsoring Space Agency
    European Space Agency (ESA)

    Sponsoring Organization
    Information Pending

    Research Benefits
    Information Pending

    ISS Expedition Duration
    September 2014 - March 2015

    Expeditions Assigned
    41/42

    Previous ISS Missions
    TripleLux-B is scheduled to operate for the first time on Increment 23/24.

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    Experiment Description

    Research Overview

    • The Gene, Immune and Cellular Responses to Single and Combined Space Flight Conditions - B (TripleLux-B) will compare the mechanisms of vertebrate and invertebrate cells at a cellular level which cause impairment of immune functions in microgravity through induction of gene activation, phagocytosis (ingestion of foreign material) and DNA repair in vertebrate and invertebrate immune cells.


    • TripleLux-B will examine the immune function of Mytilus edulis, blue mussel, hemocytes (cellular component of invertebrate blood) compared to rodent macrophages (white blood cells responsible for eating foreign material) to function in microgravity.

    Description

    Gene, Immune and Cellular Responses to Single and Combined Space Flight Conditions - B (TripleLux-B) will further the understanding of the cellular mechanisms underlying the effect of radiation responses, and the impairment of vertebrate and invertebrate immune functions in microgravity, through induction of gene activation, phagocytosis, and DNA repair.

    TripleLux-B will compare the ability of the vertebrate and invertebrate immune systems to function in microgravity. For the vertebrate portion of the study, rodent macrophages (large white blood cells) from NR8383, ATCC# CRL-2192 will be tested to determine their ability to phagocytize (ingest foreign material) zymosan (an insoluble carbohydrate that serves as an analogue of bacteria) in microgravity. For the invertebrate portion of the study the ability of Mytilus edulis, blue mussel, hemocytes (cellular component of invertebrate blood) to activate phagocytosis in microgravity will be examined.

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    Applications

    Space Applications

    Conducting studies of the immune system during space flight will provide knowledge and understanding of the effects of space habitation on the immune system. The data from these studies will be used in assessing the cellular mechanisms underlying the aggravation of radiation responses and impairment of immune functions during space flight. Understanding such risks is essential in maintaining the health and performance of crewmembers during long-duration missions.
     

    Earth Applications

    With greater understanding of the immune system in space, we can determine new countermeasures for people suffering from weakened immune systems on Earth.
     

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    Operations

    Operational Requirements

    Triplelux-B will require utilization of the MELFI and BioLab facilities onboard the ISS. To complete TripleLux-B operations two sessions of approximately 75 hours must be completed. Video containing the data of the TripleLux-B activities on orbit will be downloaded to Earth following investigation completion.

    Operational Protocols

    Prior to TripleLux-B activation the following steps must be completed by ISS crewmembers, removal of the specimen from MELFI for thawing, injection of stock culture into the culture medium, reconstitution of the stock culture in fresh medium and measurement of the specimen viability. Crewmembers will then place the specimens in two (2) BioLab Advanced Experiment Containers (AECs) for processing of approximately 75-hours. Following completion of the experiment video data collect by BioLab will be returned to Earth for analysis.

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    Results/More Information

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    Results Publications

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    Ground Based Results Publications

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    ISS Patents

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    Related Publications

      Rabbow E, Rettberg P, Baumstark-Khan C, Horneck G.  The SOS-LUX-LAC-FLUORO-Toxicity-test on the International Space Station (ISS). Advances in Space Research. 2003; 31(6): 1513-1524. DOI: 10.1016/S0273-1177(03)00086-3.
      Stojicic N, Walrafen D, Rabbow E, Baumstark-Khan C, Rettberg P, Weisshaar M, Horneck G.  Genotoxicity testing on the international space station: Preparatory work on the SOS-LUX test as part of the space experiment TRIPLE-LUX. Advances in Space Research. 2005; 36(9): 1710-1717. DOI: 10.1016/j.asr.2005.03.052.

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    Related Websites

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    Imagery

    image The Advanced Experimental Containment (AEC) hardware for the TripleLux experiments. Image courtesy of ESA.
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