Advanced Protein Crystallization Facility (APCF) - 07.29.14

Summary | Overview | Operations | Results | Publications | Imagery
ISS Science for Everyone

Science Objectives for Everyone
The Advanced Protein Crystallization Facility (APCF) was an Italian Space Agency (ASI) facility that supported a series of investigations focused on growing protein crystals in microgravity. Several investigators collaborated on the APCF, contributing samples and evaluating the facility for crystal growth in microgravity.

Science Results for Everyone
Information Pending



This content was provided by Pasquale Di Palermo, and is maintained in a database by the ISS Program Science Office.

Facility Details

OpNom:

Facility Manager(s)

  • Pasquale Di Palermo, European Space Agency (ESA), Noordwijk, Netherlands

  • Facility Representative(s)
    Information Pending
    Developer(s)

    Astrium GmbH, Immenstaad, , Germany

    Sponsoring Space Agency
    National Aeronautics and Space Administration (NASA)

    Sponsoring Organization
    Italian Space Agency (ASI)

    ISS Expedition Duration
    August 2001 - December 2001

    Expeditions Assigned
    3

    Previous ISS Missions
    The Advanced Protein Crystallization Facility flew on several space shuttle flights, beginning in 1985.

    Availability

  • Retired/Returned/Disposed
  • ^ back to top



    Facility Description

    Facility Overview

    • The Advanced Protein Crystallization Facility (APCF) was designed to produce protein crystals in microgravity.


    • The results of this study will facilitate advances in manufacturing and biological processes.
    In the Advanced Protein Crystallization Facility (APCF), crystal growth was initiated by causing a protein solution to supersaturate (a condition where there is more protein than can be dissolved in the volume of fluid). As a result of this supersaturation, the protein crystals fell out of solution and began to grow. Supersaturation was achieved by allowing water to be diffused out of the protein solution or by introducing precipitant into the solution, thereby decreasing the solubility of the protein. The APCF was the first facility to use three methods of protein crystal growth: liquid-liquid diffusion, vapor diffusion, and dialysis. During liquid-liquid diffusion, the protein solution, a buffer solution, and a precipitant solution were initially separated by shutters. When the shutters were removed, the precipitant solution diffused through the central buffer solution into the protein solution, causing the protein to become less soluble and initiating crystal growth. In the vapor diffusion method, a crystal formed in a protein solution as a precipitant drew moisture from a surrounding reservoir. In the dialysis method, salt drew moisture away from the protein solution via a membrane separating the two, forming crystals.

    Each APCF unit accommodated 48 modular protein crystal growth chambers, or reactors, of which 10 could be observed by a high-resolution video camera. The hardware consisted of a process chamber, power and data electronics, camera electronics, an optical and video system, a thermal control system, and a tape recorder. The Advanced Protein Crystallization Facility (APCF) reactors were filled in Europe and shipped to the Kennedy Space Center, Cape Canaveral, Florida, 10 days before launch. The reactors were activated after their transfer to EXpedite the PRocessing of Experiments to Space Station (EXPRESS) Rack 1 on the International Space Station. The facility's processing chamber was maintained at 20 C, and temperature data were recorded throughout the mission. Camera images in black and white were digitized and stored on the facility tape recorder. Data electronics recorded and stored other information.

    On return to Earth, the protein crystals produced in the APCF were examined using crystallography, and computers made the mathematical calculations needed to enable three-dimensional modeling of the proteins' structures.

    ^ back to top



    Operations

    Facility Operations

    • The Advanced Protein Crystallization Facility (APCF) was automated and required little crew interaction. The crew transferred the APCF hardware from the space shuttle to the International Space Station (ISS) and installed the hardware on the ISS.


    • Following APCF activation, status checks were performed daily by the crew.


    • The APCF was deactivated before it returned to Earth on the space shuttle. Samples were sent to the investigator teams for analysis.

    ^ back to top



    Results/More Information
    The initial analysis of crystals returned from the Advanced Protein Crystallization Facility indicated that the hardware performed as expected and produced high-quality protein crystals for analysis by the investigator teams.

    Results Publications

    ^ back to top


    Ground Based Results Publications

    ^ back to top


    ISS Patents

    ^ back to top


    Related Publications

    ^ back to top


    Related Websites

    ^ back to top



    Imagery

    image NASA Image: ISS003E8171 - The Advanced Protein Crystal Facility in EXPRESS Rack 1 onboard ISS Expedition 3. Two other experiments, MAMS and SAMS-II, are installed below it.
    + View Larger Image