Commercial Generic Bioprocessing Apparatus Science Insert - 03 (CSI-03) is one investigation in the CSI program series. The CSI program provides the K-12 community opportunities to utilize the unique microgravity environment of the International Space Station as part of the regular classroom to encourage learning and interest in science, technology, engineering and math. CSI-03 will examine the complete life cycle of the painted lady butterfly, eat, grow and undergo metamorphosis in space.Principal Investigator(s)
University of Colorado at Boulder, BioServe Space Technologies, Boulder, CO, United States
Baylor College of Medicine, Center for Education Outreach, Houston, TX, United States
Butterfly Pavilion, Westminster, CO, United States
Denver Museum of Nature and Science, Denver, CO, United States
Monarch Watch, Lawrence, KS, United States
National Space Biomedical Research Institute, Houston, TX, United States
National Aeronautics and Space Administration (NASA)Sponsoring Organization
National Laboratory Education (NLE)Research Benefits
Information PendingISS Expedition Duration:
October 2008 - March 2010Expeditions Assigned
18,21/22Previous ISS Missions
Space Technology and Research Students (STARSTM), a similar investigation was performed on STS-93 and STS-107. CSI-01 was begun on ISS Expedition 14 and completed during ISS Expedition 15. CSI-02 was performed during ISS Expeditions 15 - 17. CSI-03 began operation during Expedition 18.
ISS Science Challenge Selected Project
This investigation was good for us because we learned so many things we didn't know before. Here are the things we learned: We learned that a painted lady butterfly is basically a Monarch butterfly. We also learned what a pupa is and it is like a cocoon. The most interesting thing we learned is how to work as a team and how to use each other's ideas together.
- Andrea and Carlie, Grade 6, North Tama Elementary School, Traer, Iowa
Commercial Generic Bioprocessing Apparatus Science Insert - 03 (CSI-03) is one investigation in the CSI program series. The CSI program provides the K-12 community opportunities to utilize the unique microgravity environment of the International Space Station as part of the regular classroom to encourage learning and interest in science, technology, engineering and math.
CSI-03 involves several specific experiments, one will examine the complete life cycle of the Vanessa cardui, painted lady butterfly. Students will compare how the complete life cycle of the butterfly differs in space, when compared to butterflies used on Earth. The other experiment will examine the ability of an orb weaving spider to live in space. Students will compare how the spider differs in behavior, feeding and web spinning in microgravity when compared to the spiders on Earth. Another experiment will compare the ability of the older larvae of the Monarch butterfly to pupate in microgravity compared to pupation on Earth. The butterfly experiments will also compare the generalist species of butterfly larvae, painted lady butterfly, to a specialist species of butterfly larvae, Monarch butterfly.
CSI-03 is conducted in the classroom in near real-time, or it can be utilized by teachers at any point during the school year after the space flight experiments are completed. Images and data from the space-based experiments are downlinked to the BioServe Payload Operations and Control Center (POCC) on a daily basis while the experiment is active. The downlinked visual data once received will be uplinked to the BioEd Online (http://www.bioedonline.org/) website. Participating teachers will be provided with a teacher's guide featuring background information, lesson plans, and student activities for conducting the project in their classrooms.
Results from CSI-03 may help scientists more clearly understand how different organisms are affected by the microgravity environment. CSI-03 influences children to continue their education in the science, technology engineering and math areas and pursue related careers.Earth Applications
CSI-03 provides a unique educational opportunity to encourage and inspire students to pursue careers in the scientific and technical fields by participating in near real-time research activities on the ISS. This will promote education of the next generation of scientists, engineers, astronauts for the space program.
CSI-03 will operate in the CGBA under controlled temperature conditions and will require imaging and data download daily to BioServe's Payload Operations and Control Center.Operational Protocols
CSI-03 will be transferred from the Space Shuttle to the ISS and placed inside a CGBA for activation. The hardware for the experiment has been automated to allow imagery of the experiments independent of crew. The ISS crew will implement a procedure to activate CSI-03 on orbit. The ISS crew will also manipulate different feeding components of the hardware to allow fresh food or drink to be exposed for the organisms at a set point in the mission. BioServe will be able to monitor all experiments via data and image downlink. The research will be contained inside the spider and butterfly habitats.
CSI-03 investigated the growth and behavior of spiders and butterflies on board the ISS during expeditions 18 and 21/22. The experiments were designed to engage students in authentic science investigations and increase their interest in STEM academic areas. During expedition 18, CSI-03 involved more than 1,800 students comparing two orb-weaver spiders and six painted lady butterflies living on ISS to similar organisms living in ground-based habitats in classrooms. During Expedition 21/22, approximately 180,000 students from all 50 states and 23 countries were able to observe four vanessa cardui (painted lady) butterflies aboard ISS.
During expedition 18, the butterflies did not successfully pupate into adults due to food problems. The orb-weaver spiders however thrived in this environment. Students were able to track their web building and feeding activities throughout the expedition. This experiment was successful and laid the groundwork for future similar experiments to engage students. Following the completion of expedition 18, teachers evaluated their students’ interest by rating them in a range between 1 (not very interested) and 5 (very interested). The overall averages ranged between 4.6 and 4.8. CSI-03 allowed students to formulate their own questions and become personally involved in the science. Teachers also reported that 80% of students expressed interest in science careers following completion of the investigation.
On expedition 21/22, CSI-03 allowed students to track morphological, behavioral and developmental differences from their own in-class butterflies to those aboard the ISS real-time. This experiment was highly publicized by several different media sources including social media sites such as Twitter, Facebook and YouTube. In an online response survey, teachers noted that 67% of students downloaded images, 50% of students raised butterflies, 42% of students analyzed images from space, and 33% of students asked their own research questions. CSI-03 demonstrated that engaging students with hands-on scientific experiments is effective in increasing conceptual understandings and enthusiasm for science. Following CSI-03, additional space-based missions featuring spiders and plants have been performed involving thousands of students. CSI-03 has produced a model that is highly replicable and likely to appeal to a broad range of students by actively engaging them with interesting scientific topics (Moreno 2012).
Moreno NP, Vogt GL, Denk JP, Stodieck LS, Countryman S, Thomson WA. Butterflies and Spiders in Space: Space Life Science Investigations for the Classroom. Gravitational and Space Biology. 2012; 26(1): 77 - 87.