To reveal features of metabolism and morphology of different phenotypes of one strain of bifidobacteria caused by microgravity conditions to produce probiotics with the increased biomedical factors and increase the production efficiency.Principal Investigator(s)
Russian Federal Space Agency (Roscosmos)Sponsoring Organization
Information PendingResearch Benefits
Information PendingISS Expedition Duration:
March 2009 - September 2010Expeditions Assigned
19/20,21/22,23/24Previous ISS Missions
Provision of the clone collection of Bifidobacterium bifidum1 strain with heritable deviations of morphophysiological characteristics. Genetic analysis in mutant clones. Identification of viability, antagonistic activity, acid formation activity, adhesion activity, resistance to antibiotics, air oxygen, acid medium and determination of the cultivation parameters in ground conditions. Selection of possible candidates for production strains of bifidobacteria having the increased biotechnological and biomedical characteristics as compared with the wild and other production strains. Presentation of description of experiments for the characteristics analysis of A. bifidum1 strain and its changed forms in microgravity conditions and data processing.
Information PendingEarth Applications
BIF will use Case Bioecologia with ampullas sealed observing sterility containing 1.5 ml of lyophilically dried A. bifidum 1 strain in Blaurock medium with addition of protective media (sugar-gelatin and skim milk and also options with unithiol), hermetically closed test tubes containing 4 ml of liquid culture A. bifidum 1 in Blaurock medium in the concentration no less than 107 kl/ml. Thermostat Cryogem-0CI.Operational Protocols
Use of modern methods of comparative study of genetic, morphological, cytologic, biochemical and cultural characteristics of options obtained in the experiment will allow to get the maximum full characteristic of genetic and metabolic potential of Bifidobacterium bifidum 1. As a result the following is expected: a significant renewal of the spectrum of possible candidates for production strains of bifidobacteria having the increased biotechnological and biomedical characteristics as compared with a wild strain.