Commercial Generic Bioprocessing Apparatus - Kidney Cell Gene Expression (CGBA-KCGE) - 07.14.16
This investigation examined how microgravity alters the genes controlling the production of proteins in association with kidney cells; using commercial scientific hardware that has supported many biological laboratory investigations adapted to operate in space and which require temperature controls from 4°C to 37°C. This research will allow the manipulation of cells to produce specific tissues for use as models in the development of new medicines to treat kidney disorders. This investigation was part of a suite of three experiments that used the CGBA hardware. Science Results for Everyone
This investigation examined effects of spaceflight on genes controlling protein production in kidney cells. Previous work showed alterations in kidney cell genes in microgravity and demonstrated that cells in space form 3-dimensional structures similar to those found in organs (and extremely difficult to duplicate on Earth). Ultimately, this research could lead to tissue models for developing new medicines. Post-flight analysis indicated that, although the bioprocessing hardware operated properly, unexpected drifts above planned temperatures occurred in two of seven containers. Ground tests were completed for comparison to in-flight samples, but final data analysis has not yet been released. Experiment Details
Timothy G. Hammond, M.B.B.S., Durham Veterans' Affairs Medical Center, Durham, NC, United States
BioServe Space Technologies, University of Colorado, Boulder, CO, United States
Sponsoring Space Agency
National Aeronautics and Space Administration (NASA)
Human Exploration and Operations Mission Directorate (HEOMD)
ISS Expedition Duration
September 2000 - November 2000
CGBA-KCGE has previously flown on STS-90.
- Cellular models are needed in the development of new medicines to treat various kidney diseases. Previous research has shown that renal (kidney) cells grown in microgravity produce three-dimensional structures that are similar to how tissues are formed in the human body on Earth.
- CGBA-KCGE examined the effects of spaceflight on genes that control protein production in kidney cells.
- Ultimately, this research will allow the manipulation of cells to produce specific tissues for use as models in the development of new medicines to treat kidney disorders.
Observing the effects that microgravity has on cellular structures, gives insight into how the human body reacts at the cellular level. These changes may lead to the development of countermeasures that would lower the human risk of long duration spaceflight.
Manipulating cells samples to create tissues that are similar in structure to that of those found in the human body can be used in many types of research applications. These samples can be used for developing new medications to treat various diseases.
Operational Requirements and Protocols
Decadal Survey Recommendations
Information Pending^ back to top
Preliminary results indicated that that an average of 60% of the kidney cell samples from CGBA-KCGE were drawn into the Vacutainers. Although the sample size was smaller, the samples were sufficient for postflight analysis. For the synaptogenesis experiment with fruit flies, preliminary results indicated that although the CGBA hardware operated successfully, there were unexpected temperature drifts above the planned temperature in two of the seven containers. While ground tests were completed for comparison to the in-flight samples, final data analysis has not been released. (Evans et al. 2009)^ back to top
Ground Based Results Publications
A Modular Suite of Hardware Enabling Space Flight Cell Culture Research - Commercial Generic Bioprocessing Apparatus (CGBA)
BioServe Space Technologies
NASA Fact Sheet
Commercial Generic Bioprocessing Apparatus (CBGA) Isothermal Containment Module version 3 (ICM v.3) with eight containers. The ICM v.3 provides the computer and thermal control for the samples. Courtesy image of Colorado University.
+ View Larger Image