Study of Microbial Communities Exposed to Weightlessness (Sample) will show how microbes are carried into space by humans and end up on surfaces and equipment in the International Space Station (ISS), as well as how microbes survive, adapt and possibly multiply on ISS. Sample will also indicate if there are "hot spots" (places where microbes grow most easily) on ISS; this will make it possible to take appropriate measures to keep ISS clean and the crewmembers healthy.Principal Investigator(s)
Dutch Space, Leiden, , Netherlands
European Space Agency (ESA)Sponsoring Organization
Information PendingResearch Benefits
Information PendingISS Expedition Duration:
October 2003 - April 2007Expeditions Assigned
8,9,12,14Previous ISS Missions
The Sample investigation has been performed on ISS Expeditions 8 and 11.
Sample will evaluate which microbial species might benefit from growth conditions on board the International Space Station (ISS). This investigation will focus on potentially pathogenic and destructive microbes to determine the origin and distribution of species on different sample sites. To complete the Sample investigation the changes of normal microbiota (microbes of an area) of crewmembers and the relation with the microbiota in the ISS during space flight will be determined. Sample will investigate the mechanism of microbial adaptation to microgravity and study if there is microbial adaptation to weightlessness. Sample will also determine how exposure to weightlessness can affect adhesion properties of microorganisms.
Based on Sample results, a machine may be designed that can monitor microbial growth in space, without sending samples to Earth. Developers of future habitability procedures can use the knowledge gained from this experiment to better protect crew health on long duration missions.Earth Applications
Sample will generate knowledge and tools to investigate hygienic conditions in hospitals. Sample techniques, are currently being used to analyze the spread of microbes in intensive care units (ICUs), with special attention to methicillin-resistant Staphylococcus aureus (MRSA).
From several selected sites of the ISS, duplicate samples are collected. The samples are taken by rubbing swab sticks over a defined surface. The sampling sites include buttons, keyboards, personal care appliances; places where there is a large amount of contact between crew and surface. Furthermore, wall panels and other places where condensation creates moist surfaces are sampled. In addition, electrical wires that produce some heat that might favor microbial growth and places with visible microbial growth are sampled. Samples of various body parts are taken from one of the crewmembers. As a ground control, similar samples are taken during a test or training period in which the cosmonaut is exposed to a large amount of stress. This is to evaluate normal fluctuation of the microbiota on Earth under stress conditions.Operational Protocols
Samples of ISS and resident crewmembers are taken for further culturing and analysis on Earth. These samples need to be placed in a protective gel medium to keep microbes alive. From these samples, bacterial strains will be isolated on Earth and screened for unusual adhesion abilities. To understand if exposure to weightlessness can affect adhesion properties of microorganisms, experiments are carried out using cultures of a model bacterium, Escherichia coli. The bacteria are grown in liquid medium under weightless conditions. In these conditions, either physiological adaptation of the culture or insurgence of mutations in the bacterial population might take place. Either event might lead to increased biofilm formation compared to that observed for the same Escherichia coli strain growth in the same conditions on Earth, in normal gravity conditions. Scientist's will then analyze and characterize the precise molecular event leading to biofilm formation.