NanoRacks-CellBox-Effect of Microgravity on Human Thyroid Carcinoma Cells (NanoRacks-CellBox-Thyroid Cancer) - 07.15.14

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NanoRacks-CellBox-Effect of Microgravity on Human Thyroid Carcinoma Cells (NanoRacks-CellBox-Thyroid Cancer) investigates the effects of microgravity on human thyroid carcinoma cells. The overall aim of the studies in microgravity is the identification of new biomarkers which can be used for developing new cancer drugs.

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The following content was provided by Daniela Gabriele Grimm, MD, and is maintained in a database by the ISS Program Science Office.

Experiment Details

OpNom BioRack Experiment Containers

Principal Investigator(s)

  • Daniela Gabriele Grimm, MD, Aarhus University, Aarhus, Denmark

  • Co-Investigator(s)/Collaborator(s)
  • Jessica Pietsch, Ph.D., Otto-von-Guericke-University Magdeburg, Magdebug, Germany
  • Stefan Riwaldt, Universit√§tsklinikum Magdeburg, Magdebug, Germany
  • Markus Wehland-von Trebra, Ph.D., Otto-von-Guericke-University Magdeburg, Magdebug, Germany

  • Developer(s)
    EADS ASTRIUM, Friedrichshafen, , Germany

    NanoRacks, LLC, Houston, TX, United States

    Sponsoring Space Agency
    National Aeronautics and Space Administration (NASA)

    Sponsoring Organization
    National Laboratory (NL)

    Research Benefits
    Scientific Discovery, Space Exploration, Earth Benefits

    ISS Expedition Duration
    March 2014 - September 2014

    Expeditions Assigned
    39/40

    Previous ISS Missions
    Information Pending

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    Experiment Description

    Research Overview

    • NanoRacks-CellBox-Effect of Microgravity on Human Thyroid Carcinoma Cells (NanoRacks-CellBox-Thyroid Cancer) investigates the effects of microgravity on human thyroid cancer cells.

    • The goal is to possibly identify new biomarkers in the genome (hereditary information), the proteome (expressed proteins) or the secretome (secreted proteins) which could be used to develop new thyorid cancer drugs or to gain a better understanding of the mechanism leading to cancer development and thus to new strategies of thyroid cancer therapy.

    Description

    The principle aim of NanoRacks-CellBox-Effect of Microgravity on Human Thyroid Carcinoma Cells (NanoRacks-CellBox-Thyroid Cancer) is to investigate the effects of real microgravity on human thyroid carcinoma cells (FTC-133 cell line). Data obtained from this experiment is compared to the thyroid cancer cells characterized after exposure to simulated microgravity and after the successful SIMBOX on Shenzhou-8 mission (2011, Experiment 14).

     

    Single cancer cells of the FTC-133 cell line are cultured during NanoRacks-CellBox-Thyroid Cancer) investigation. The cells are expected to form three-dimensional multicellular tumor spheroids (MCTS), which clearly resemble the respective originating tumor. Under conditions of microgravity, cells keep floating without stirring so that initial cell-cell interactions required for spheroid formation are induced by forces only due to biochemical components actually expressed on surfaces of cells but gravity related push and shear events do not influence MCTS formation. After the SIMBOX mission, it was shown for the first time that FTC-133 cells grew in form of 3D multicellular spheroids and also adherently as a monolayer in space. Epidermal growth factor (EGF) and connective tissue growth factor (CTGF) may be involved in 3D aggregation in space. The secretion of cytokines was down-regulated in space. The inducers of tumor neoangiogenesis OPN, IL-6, IL-8, and VEGF-a were all reduced under real microgravity. Several gravisensitive signaling elements, such as protein kinases A and C as well as integrins, are involved in the reaction of thyroid cancer cells to microgravity. The increase in VEGF-d secretion of the original low differentiated thyroid cancer cells indicated a redifferentiation of these cells in space. The hope is to confirm these findings and thus increase the number of experiments for statistical reasons. The overall aim of the studies in microgravity is the identification of new biomarkers which can be used for developing new cancer drugs.

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    Applications

    Space Applications
    Information Pending

    Earth Applications

    This investigation may reveal new biomarkers, which could be used to develop new thyroid cancer drugs.

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    Operations

    Operational Requirements

    BioRack Experiment Containers are returned at 4°C.

    Operational Protocols

    A crewmember installs BioRack Experiment Containers no later than docking plus two days. After automatic fixation, the containers must remain in the BioRack for a minimum of 12 days prior to the crewmember removing and storing at 4°C for return.

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    Results/More Information
    Information Pending

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    Related Websites
    NanoRacks

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    Imagery