The High Quality Protein Crystal Growth Experiment (JAXA PCG) is aimed at the growth of crystals of biological macromolecules by the counter-diffusion technique. The main scientific objective of the JAXA PCG experiment is to make the fine quality protein crystals under microgravity environment. And the space-grown crystals will be applied structural biology and pharmaceutical activity.Principal Investigator(s)
Japan Aerospace Exploration Agency (JAXA), Tsukuba, , Japan
Japan Aerospace Exploration Agency (JAXA)Sponsoring Organization
Information PendingResearch Benefits
Information PendingISS Expedition Duration:
March 2009 - September 2014Expeditions Assigned
19/20,21/22,23/24,27/28,29/30,33/34,35/36,37/38,39/40Previous ISS Missions
The precursor to JAXA PCG, JAXA GCF was completed in Russian Service Module from 2003 to 2008.
JAXA has developed a new technique that estimates the driving force ratio of crystals grown on the ground and in space, and the capture ratio of impurities through the diffusion/capture coefficient of protein. This technique proved that under microgravity conditions with high viscosity and slow diffusion, a protein depletion zone and impurity depletion zone will appear. Using this technique we try to get high quality protein crystals. Our goal is to contribute to yielding results which meet the social requirements.
This experiment will contribute to the society by creating new drugs for diseases and new catalyst for environment or energy. That will show the effecitivity of ISS.Earth Applications
This experiment will contribute to the society by creating new drugs for diseases and new catalyst for environment or energy.
Protein crystallization experiment in space environment has been performed for more than 20 years. JAXA has conducted protein crystallization experiment in ISS since 2003. In this experiment, totally over 300 protein samples were launched by?Russian space transportation system and JAXA developed the technology to obtain the high quality crystals in space. JAXA also established user-friendly support service system for users to apply to the space experiment easily.About 80% proteins were crystallized in past JAXA PCG mission. This ratio is almost equal to the past protein crystallization experiment of JAXA.The excellent diffraction data to be able to analyze the protein structure newly was obtained for three kinds of proteins. Now detaled structure analysis of those proteins are conducted. The space-grown crystals will be applied structural biology and pharmaceutical activity.
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Inaka K, Takahashi S, Aritake K, Tsurumura T, Furubayashi N, Yan B, Hirota E, Sano S, Sato M, Kobayashi T, Yoshimura Y, Tanaka H, Urade Y. High-Quality Protein Crystal Growth of Mouse Lipocalin-Type Prostaglandin D Synthase in Microgravity. Crystal Growth and Design. 2011; 11(6): 2107-2111. DOI: 10.1021/cg101370v.
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