RNA Interference and Protein Phosphorylation in Space Environment Using the Nematode Caenorhabditis elegans (CERISE) - 12.03.13
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RNA Interference and Protein Phosphorylation in Space Environment Using the Nematode Caenorhabditis elegans (CERISE) evaluates the effect of microgravity on RNA interference and studies how the space environment effects protein phosphorylation and signal transduction in the muscle fibers of Caenorhabditis elegans.
Science Results for Everyone
Japan Aerospace Exploration Agency (JAXA)Sponsoring Organization
Information PendingResearch Benefits
Information PendingISS Expedition Duration
October 2009 - March 2010Expeditions Assigned
21/22Previous ISS Missions
The predecessor to this investigation, ICE-First, was flown to ISS in 2004.
- RNA interference is an useful technique to artificially knock down a target gene expression. In this experiment we will examine RNA interference under space environment. We will also evaluate the effect of space environment on protein phosphorylation and signal transduction concerning muscle fibers formation using gene knock-downed C.elegans.
Examine RNA interference under space environment and evaluate effect of space environment for protein phosphorylation and signal transduction concerning muscle fibers formation using gene knock downed C.elegans
Information PendingEarth Applications
The experimental data will provide the important information to clarify the muscle atrophy in space and also on the Earth. The results will be the first evidence that RNA interference will be a powerful technique in space experiment to investigate the effect of microgravity on gene expression.
Information PendingOperational Protocols
Etheridge T, Nemoto K, Hashizume T, Mori C, Sugimoto T, Suzuki H, Fukui K, Yamazaki TQ, Higashibata A, Szewczyk NJ, Szewczyk NJ, Higashitani A, Higashitani A.The next phase of life-sciences spaceflight research: Harnessing the power of functional genomics.Communicative and Integrative Biology. 2011 Dec; 4(6): 668-669.
Etheridge T, Nemoto K, Hashizume T, Mori C, Sugimoto T, Suzuki H, Fukui K, Yamazaki TQ, Higashibata A, Szewczyk NJ, Szewczyk NJ, Higashitani A, Higashitani A. The Effectiveness of RNAi in Caenorhabditis Elegans Is Maintained During Spaceflight.PLOS ONE. 2011 06/01/2011; 6(6): e20459. DOI: 10.1371/journal.pone.0020459.
Ground Based Results Publications
Higashitani A, Higashitani A, Hashizume T, Sugimoto T, Mori C, Nemoto K, Etheridge T, Higashitani N, Takanami T, Suzuki H, Fukui K, Yamazaki TQ, Ishioka N, Szewczyk NJ, Szewczyk NJ, Higashibata A. C. elegans RNAi space experiment (CERISE) in Japanese Experiment Module KIBO. Biological Sciences in Space. 2009; 23(4): 183-187. DOI: 10.2187/bss.23.183.