Dome Gene Experiment (DomeGene) - 12.03.13
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Japan Aerospace Exploration Agency (JAXA)Sponsoring Organization
Information PendingResearch Benefits
Information PendingISS Expedition Duration
October 2008 - April 2009Expeditions Assigned
18Previous ISS Missions
Two kinds of amphibian cultured cell lines are cultured in Dome Gene. A-6 cell line derived from kidney and A-8 cell line derived from liver are used. They show different types of cell differentiation and morphogenesis. While they are cultured under microgravity,we observe the shape and state of the cells. And, we examine the known and unknown gene expression by DNA array assay using fixed and frozen recovery sample.
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Information PendingOperational Protocols
Kitamoto J, Fukui A, Asashima M. Temporal regulation of global gene expression and cellular morphology in Xenopus kidney cells in response to clinorotation. Advances in Space Research. 2005 January; 35(9): 1654-1661.
Ikuzawa M, Akiduki S, Asashima M. Gene expression profile of Xenopus A6 cells cultured under random positioning machine shows downregulation of ion transporter genes and inhibition of dome formation. Advances in Space Research. 2007 January; 40(11): 1694-1702. DOI: 10.1016/j.asr.2007.08.014.
Ikuzawa M, Asashima M. Global Expression of Simulated Microgravity-Responsive Genes in Xenopus Liver Cells.Zoological Science. 2008 August; 25(8): 828-837. DOI: 10.2108/zsj.25.828.
Ground Based Results Publications
Kidney Cell on the left cultured in a microgravity environment. Kidney cells on the right cultured in an artificial gravity environment. Image courtesy of Japan Aerospace Exploration Space Agency (JAXA).
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