NanoRacks-Protein Crystal Growth-1 (NanoRacks-PCG-1) - 09.17.14

Overview | Description | Applications | Operations | Results | Publications | Imagery
ISS Science for Everyone

Science Objectives for Everyone
NanoRacks-Protein Crystal Growth-1 (NanoRacks-PCG-1) is a proprietary protein crystal growth experiment that utilizes state-of-the-art on-the-ground PCG procedures and hardware.

Science Results for Everyone

NanoRacks-PCG-1 uses commercial, off-the shelf, state-of-the-art way to grow protein crystals in microgravity using small slides known as CrystalCards ™. The slides are launched frozen, thawed on orbit to allow crystal growth, examined while on-orbit, and then returned to Earth. After 70 days on the station, 16 of the 25 cards, or 64 percent, had crystals, compared to 12 of 25 in the ground controls, or 48 percent. More high-quality crystals grew in the microgravity cards than the ground controls as well. These results validate use of the system, and provide new opportunities for large-protein crystal growth that does not grow as well back on Earth.



The following content was provided by Carl W. Carruthers, Jr., M.S., and is maintained in a database by the ISS Program Science Office.

Experiment Details

OpNom NanoRacks Module-19

Principal Investigator(s)

  • Carl W. Carruthers, Jr., M.S., Houston, TX, United States

  • Co-Investigator(s)/Collaborator(s)
    Information Pending
    Developer(s)
    NanoRacks, LLC, Webster, TX, United States

    Sponsoring Space Agency
    National Aeronautics and Space Administration (NASA)

    Sponsoring Organization
    National Laboratory (NL)

    Research Benefits
    Information Pending

    ISS Expedition Duration
    September 2012 - September 2013

    Expeditions Assigned
    33/34,35/36

    Previous ISS Missions
    Information Pending

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    Experiment Description

    Research Overview

    • NanoRacks-Protein Crystal Growth-1 (NanoRacks-PCG-1) utilizes different Protein Crystal Growth solutions in small crystal slides to grow protein crystals in microgravity.
    • The slides are launched frozen, thawed on orbit to allow crystal growth, examined while on-orbit and then returned.

    Description

    NanoRacks-Protein Crystal Growth-1 (NanoRacks-PCG-1) is a proprietary protein crystal growth experiment that utilizes state-of-the-art on-the-ground PCG procedures and hardware.  NanoRacks-PCG-1 uses different PCG solutions in small crystal slides to grow protein crystals in microgravity. The slides are launched frozen, thawed on orbit to allow crystal growth, examined while on-orbit and then returned.

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    Applications

    Space Applications

    The applications of this investigation are proprietary.

    Earth Applications

    The applications of this investigation are proprietary.

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    Operations

    Operational Requirements

    The protein growth in the Crystal Plates automatically initiates on-orbit when the Plates thaw. At least one analysis session with the Crystal Plates and the NanoRacks Microscope-2 facility is conducted to collect data on crystal growth.  After the session is completed, the Crystal Plates are re-stowed.

    Operational Protocols

    NanoRacks Module-19 is soft-stowed in a Glacier at -95°C.  Crew removes the module from Glacier and places it inside NanoRacks Platform-1.  Thaw and crystal growth occur without crew interaction.  The crew observes crystal growth status on the slides.  

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    Results/More Information
    Information Pending

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    Results Publications

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    Ground Based Results Publications

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    ISS Patents

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    Related Publications

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    Related Websites
    NanoRacks

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    Imagery

    image
    Lysozyme (above) and Thermolysin (below) crystals grown in microgravity on the ISS. In collaboration with Carl Carruthers at Houston Methodist Research Institute, NanoRacks developed a new method of growing microgravity protein crystals, allowing researchers the possibility to grow more crystals with very little protein. Image courtesy of Carl Carruthers.

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    image

    NanoRacks-Protein Crystal Growth-1 (NanoRacks-PCG-1) is a proprietary protein crystal growth housed inside NanoRacks Module-19.  Image courtesy of Carl W. Carruthers, Jr.


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