Transgenic Arabidopsis Gene Expression System - Intracellular Signaling Architecture (APEX-03-2 TAGES-Isa) - 12.10.14

Overview | Description | Applications | Operations | Results | Publications | Imagery
ISS Science for Everyone

Science Objectives for Everyone
On Earth, plants use gravity, moisture and light to determine which way to grow, but the microgravity environment of space causes them to develop different growth habits. The Transgenic Arabidopsis Gene Expression System - Intracellular Signaling Architecture (APEX-03-2 TAGES-Isa) investigation studies thale cress (Arabidopsis thaliana) seedlings grown in microgravity, examining the molecular changes that affect their growth. Results provide new insight into how plants respond to extraterrestrial environments, which improves the research for growing food and producing oxygen on future space missions.

Science Results for Everyone
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The following content was provided by Robert J. Ferl, Ph.D., and is maintained in a database by the ISS Program Science Office.

Experiment Details

OpNom APEX-03-2 TAGES-Isa

Principal Investigator(s)

  • Robert J. Ferl, Ph.D., University of Florida, Gainesville, FL, United States

  • Co-Investigator(s)/Collaborator(s)
  • Anna-Lisa Paul, Ph.D., University of Florida, Gainesville, FL, United States

  • Developer(s)
    Kennedy Space Center, , FL, United States

    Sponsoring Space Agency
    National Aeronautics and Space Administration (NASA)

    Sponsoring Organization
    Human Exploration and Operations Mission Directorate (HEOMD)

    Research Benefits
    Scientific Discovery, Space Exploration

    ISS Expedition Duration
    September 2014 - March 2015

    Expeditions Assigned
    41/42

    Previous ISS Missions
    TAGES-ISA builds upon the previously flown Plant Growth Investigations in Microgravity (PGIM) experiment which flew on STS-93, the Biological Research in Canisters (BRIC)-16 experiment which flew on STS-131, and the Transgenic Arabidopsis Gene Expression System (TAGES) experiment which flew in ABRS during Increments 19-24.

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    Experiment Description

    Research Overview

    • Plants experiencing spaceflight are quite normal in appearance but can exhibit growth habits distinctly different from plants on earth. This research explores the molecular biology guiding the altered growth of plants in spaceflight.
       
    • TAGES-Isa specifically addresses the growth and molecular changes that occur in Arabidopsis thaliana plants during spaceflight. By using molecular and genetic tools, fundamental questions regarding root structure, growth and cell wall remodeling may be answered.
       
    • This investigation advances the fundamental understanding of the molecular biological responses to extraterrestrial environments. This understanding helps to further define the impacts of spaceflight on biological systems to better enable NASA’s future space exploration goals. 

    Description

    Plants experiencing spaceflight are quite normal in appearance, but can exhibit growth habits distinctly different from plants on earth. Historically, the spaceflight-induced differences were difficult to dissect due to changing hardware and flight conditions. With the ABRS and a consistent ISS orbital environment, it is now possible to confidently approach a dissection of the molecular biology guiding the altered growth of plants in spaceflight. TAGES-ISA involves experiments that specifically address growth and molecular changes in Arabidopsis that occur during spaceflight, bringing the molecular and genetic tools of Arabidopsis to bear on fundamental questions of root morphology, growth, and cell wall remodeling. During the APEX-TAGES experiments, a remarkable number of gene expression changes during spaceflight were observed that are associated with cell wall restructuring, especially in roots. It was also observed, with the ABRS/Green Fluorescent Protein (GFP) imaging system, genetically dependent changes in root growth direction, movement, and structure. The current experiments employ the ABRS and the GFP Imager to dissect, and better understand the molecular changes guiding growth restructuring on orbit. In particular it is hypothesized that spaceflight conditions engender a constant state of root cell wall modification during the cell file rotations that characterize spaceflight-induced root skewing. The specific experiments involve mutants in root growth and cell wall signaling pathways identified in previous spaceflight molecular studies, as well as new GFP biosensor lines also based on the genes identified in the previous flight experiments.

    The overall goal of the program is to understand fundamental molecular biological responses to extraterrestrial environments. This goal aligns with fundamental space biology goals to understand the impact of spaceflight on biological systems to better enable the exploration imperative. The research team has used molecular biology and genetically tagged plants as biological monitors of spaceflight and space-related environments with great science return, leading to the development of new biological and hardware tools to study the spaceflight response with even higher fidelity in a wider variety of spaceflight-related environments. The focus of the current proposal is to build on these data and other insights gained from the execution of the APEX-TAGES experiments in the ABRS/GFP Imager, advancing science while also advancing the telemetric imaging hardware and biological experiment support for the ISS and future platforms. Gaining insight into such mechanisms is recognized as fundamental within the decadal study and underpin answers to some of the biggest questions in spaceflight plant biology.

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    Applications

    Space Applications

    Plants grown in space are different from those grown on Earth, yet these differences have been hard to study, because each spaceflight is different and experimental hardware continually changes. Previous investigations by the TAGES-ISA team pioneered methods to harvest and preserve plant specimens in space, and the investigation builds on previous plant growth experiments, providing a stronger comparison for plant biologists. Results from this investigation improve scientists’ understanding of the cellular and molecular changes taking place in plants grown in space. This provides fundamental insight for future missions, including efforts to grow food for journeys beyond low-Earth orbit.

    Earth Applications

    Understanding how plants change in response to their environments provides fundamental insight into plant biology. Results from this investigation have implications for improving agricultural and biomass production, benefiting people on Earth.

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    Operations

    Operational Requirements

    The time between launch and Run 1 installation into ABRS GFP Imager is 5-12 days. The experiment run duration is 10-12 days. The previous run harvest and next run installation should be scheduled on the same day. The time between harvest and MELFI insertion is 24 hours. Downlink of GFP images is required daily. Harvest photos are required; live video of harvest operations is requested when possible. Only the Water Refill Kit and KFTs return to Earth. KFTs return at -20°C in Cold Bag.

    Operational Protocols

    ABRS is prepared for the TAGES-ISA experiment by filling the ABRS water reservoir and installing the ABRS Air Filter Cartridge. To start Run 1 of the TAGES-Isa experiment, six petri plates are removed from a Nomex bag and transferred to the ABRS GFP Imager. GFP and white light images is downlinked on a daily basis. After an experiment duration of 10-12 days, the GFP Imager is removed from ABRS and transferred to the Maintenance Work Area for the harvest activity. Each petri plate is removed from the GFP Imager, photographed, and harvested. The harvest activity requires the crewmember to use forceps to pull the plants from the agar surface on the petri plate. The plants are placed into a KFT and actuated to deliver the RNALater chemical preservative to the plants. The harvest procedure is completed for each of the six petri plates. Upon completion of the harvest, the petri plates for the next run are inserted into the GFP Imager, and the GFP Imager is transferred back to ABRS. Twenty-four hours following the harvest, the KFTs are transferred to MELFI at -80°C. A total of three harvests are completed. At the conclusion of the final harvest, a desiccant pack is inserted into the GFP Imager, the ABRS water reservoir drained, ABRS is powered down, and the Water Refill Kit and APEX-03 Science Spares Kit are returned at ambient, and the KFTs return at -20°C.

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    Results/More Information
    Information Pending

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    Results Publications

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    Ground Based Results Publications

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    ISS Patents

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    Related Publications

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    Related Websites

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    Imagery