Question 4. Audio transcript, Mike Flynn describes a simple test that illustrates how the "lab on a CD" works.
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Full Transcript (below)
4. Please describe a simple test with the CDs and dyes that finds live and dead bacteria levels in a sample. (1:02 MINUTES)
"Ah, now, there’s two dyes that are used. One dye will flow easily through the membrane of the cells and go in and bind to the DNA. And when that occurs, it gives off a green light. The second stain cannot get through the membrane of the cell. It can only get through the membrane of the cell if the membrane of the cell is broken or ruptured, and in that case, it will go in, and it will bind to the DNA, and it will produce a red color. So, if the cell is alive, and the membrane is still good, it’s gonna fluoresce green. It’s gonna give off green light, and if the cell is dead, it’s gonna give off red light. And you can use that to discriminate between the live and the dead bacteria. And then you can just add up all the li .. all the red and green stained cells to determine what the total number of cells are in the sample. So, based on that, you can determine how many bacteria are in this drop of water and how many of those bacteria are alive, and how many of those bacteria are dead.”
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